Simple sequence repeats in Cucumis mapping and map merging

被引:75
作者
Danin-Poleg, Y
Reis, N
Baudracco-Arnas, S
Pitrat, M
Staub, JE
Oliver, M
Arus, P
deVicente, CM
Katzir, N
机构
[1] Agr Res Org, Newe Yaar Res Ctr, Dept Vegetable Crops, IL-30095 Ramat Yishay, Israel
[2] INRA, Stn Genet & Ameliorat Fruits & Legumes, F-84143 Montfavet, France
[3] Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Unit, Madison, WI 53706 USA
[4] Inst Recerca & Technol Agroalimentaires, Dept Genet Vegetal, Cabrils 08340, Spain
关键词
microsatellites; Cucumis melo; melon; Cucumis sativus; cucumber; comparative mapping;
D O I
10.1139/gen-43-6-963
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Thirty-four polymorphic simple-sequence repeats (SSRs) were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (Cucumis sativus L.). SSR markers were located on three melon maps (18 on the map of 'Vedrantais' and PI 161375, 23 on the map of 'Piel de Sapo' and PI 161375, and 16 on the map of PI 414723 and 'Dulce'). In addition, 14 of the markers were located on the cucumber map of GY14 and PI 183967. SSRs proved to be randomly distributed throughout the melon and cucumber genomes. Mapping of the SSRs in the different maps led to the cross-identification of seven linkage groups in all melon maps. In addition, nine SSRs were common to both melon and cucumber maps. The potential of SSR markers as anchor points for melon-map merging and for comparative mapping with cucumber was demonstrated.
引用
收藏
页码:963 / 974
页数:12
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