Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

被引：39

作者：

Amann, G

Stetter, KO ^{[1
]}

Llobet-Brossa, E

Amann, R

Antón, J

机构：

[1] Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany

[2] Max Planck Inst Marine Microbiol, Bremen, Germany

[3] Univ Alicante, Div Microbiol, E-03080 Alicante, Spain

来源：

EXTREMOPHILES | 2000年 / 4卷 / 06期

关键词：

16S rDNA heterogeneity; Archaea; Haloarcula marismortui; fluorescence in situ hybridization;

D O I：

10.1007/s007920070007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

引用

页码：373 / 376

页数：4

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