Root-Specific Transcript Profiling of Contrasting Rice Genotypes in Response to Salinity Stress

被引:111
作者
Cotsaftis, Olivier [1 ]
Plett, Darren [1 ]
Johnson, Alexander A. T. [1 ]
Walia, Harkamal [2 ]
Wilson, Clyde [3 ]
Ismail, Abdelbagi M. [4 ]
Close, Timothy J. [2 ]
Tester, Mark [1 ]
Baumann, Ute [1 ]
机构
[1] Australian Ctr Plant Funct Genom, Glen Osmond, SA 5064, Australia
[2] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA
[3] USDA ARS, Salin Lab, Riverside, CA 92507 USA
[4] Int Rice Res Inst, Manila 1099, Philippines
基金
澳大利亚研究理事会;
关键词
Rice; root; salinity tolerance; microarray; HKT; Saltol; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE GENE; SALT TOLERANCE; ARABIDOPSIS-THALIANA; PHYSIOLOGICAL CHARACTERIZATION; ALANINE-AMINOTRANSFERASE; POLYAMINE METABOLISM; PROLINE BIOSYNTHESIS; CATION TRANSPORTERS; EXPRESSION ANALYSIS; HKT TRANSPORTERS;
D O I
10.1093/mp/ssq056
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Elevated salinity imposes osmotic and ion toxicity stresses on living cells and requires a multitude of responses in order to enable plant survival. Building on earlier work profiling transcript levels in rice (Oryza sativa) shoots of FL478, a salt-tolerant indica recombinant inbred line, and IR29, a salt-sensitive cultivar, transcript levels were compared in roots of these two accessions as well as in the roots of two additional salt-tolerant indica genotypes, the landrace Pokkali and the recombinant inbred line IR63731. The aim of this study was to compare transcripts in the sensitive and the tolerant lines in order to identify genes likely to be involved in plant salinity tolerance, rather than in responses to salinity per se. Transcript profiles of several gene families with known links to salinity tolerance are described (e.g. HKTs, NHXs). The putative function of a set of genes identified through their salt responsiveness, transcript levels, and/or chromosomal location (i.e. underneath QTLs for salinity tolerance) is also discussed. Finally, the parental origin of the Saltol region in FL478 is further investigated. Overall, the dataset presented appears to be robust and it seems likely that this system could provide a reliable strategy for the discovery of novel genes involved in salinity tolerance.
引用
收藏
页码:25 / 41
页数:17
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