The comparative behavior of Lactococcus lactis in free and immobilized culture processes

被引：14

作者：

Cachon, R ^{[1
]}

Antérieux, P ^{[1
]}

Diviès, C ^{[1
]}

机构：

[1] Univ Bourgogne, ENSBANA, UA INRA, Microbiol Lab, F-21000 Dijon, France

来源：

JOURNAL OF BIOTECHNOLOGY | 1998年 / 63卷 / 03期

关键词：

culture processes; growth; LDH activity; Lactococcus; NADH/NAD ratio; pH; intracellular;

D O I：

10.1016/S0168-1656(98)00083-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study presents the changes in the physiological state of Lactococcus lactis in different actively growing systems. The intracellular pH (pH(in)), NADH/NAD ratio and lactate dehydrogenase (LDH) activity were measured in free cell batch culture with or without external pH (pH(out)) control, and in continuous free or immobilized cell reactors. In free cell batch culture it has been shown that the pH(in) is dependent both on pH(out) and age of the culture, while in free cell continuous culture the pH(in) and the pH(out) are close together. The NADH/NAD ratio varied inversely with the growth rate, which was related to the changes in the metabolic activities in lactococci. The specificity of the immobilized cell technology has been characterized, the properties of realized cells followed those of immobilized cells. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：211 / 218

页数：8

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