Usefulness of a quick decalcification of bone sections embedded in methyl metacrylate: an improved method for immunohistochemistry

被引：33

作者：

Gomes, Samirah Abreu ^{[1
]}

dos Reis, Luciene Machado ^{[2
]}

de Oliveira, Ivone Braga ^{[2
]}

Noronha, Irene de Lourdes ^{[2
]}

Jorgetti, Vanda ^{[2
]}

Heilberg, Ita Pfeferman ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Div Nephrol, BR-04023062 Sao Paulo, Brazil

[2] Univ Sao Paulo, Div Nephrol, Sao Paulo, Brazil

来源：

JOURNAL OF BONE AND MINERAL METABOLISM | 2008年 / 26卷 / 01期

关键词：

bone; immunohistochemistry; methyl methacrylate; osteoprotegerin; chronic kidney disease;

D O I：

10.1007/s00774-007-0788-2

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Immunohistochemistry of undecalcified bone sections embedded in methyl methacrylate (MMA) is not commonly employed because of potential destruction of tissue antigenicity by highly exothermic polymerization. The aim of the present study was to describe a new technique in which a quick decalcification of bone sections embedded in MMA improves the results for immunohistochemistry. The quality of interleukin 1 alpha (IL-1 alpha) immunostaining according to the present method was better than the conventional one. Immunostaining for osteoprotegerin (OPG) and the receptor activator of NF-kappa B ligand (RANKL) in bone sections of chronic kidney disease patients with mineral bone disorders (CKD-MBD) was stronger than in controls (postmortem healthy subjects). The present study suggested that this method is easy, fast, and effective to perform both histomorphometry and immunohistochemistry in the same bone fragment, yielding new insights into pathophysiological aspects and therapeutic approaches in bone disease.

引用

页码：110 / 113

页数：4

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