CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

被引：482

作者：

Liu, Hao ^{[1
,2
]}

Ding, Yuduan ^{[1
,2
]}

Zhou, Yanqing ^{[2
]}

Jin, Wenqi ^{[2
]}

Xie, Kabin ^{[3
]}

Chen, Ling-Ling ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Coll Informat, Agr Bioinformat Key Lab Hubei Prov, Wuhan 430070, Peoples R China

[3] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

来源：

MOLECULAR PLANT | 2017年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

GUIDE RNA; DESIGN; SGRNAS; SYSTEM; CAS9;

D O I：

10.1016/j.molp.2017.01.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：530 / 532

页数：3

共 14 条

[1] Microhomology-based choice of Cas9 nuclease target sites [J].

Bae, Sangsu ;

Kweon, Jiyeon ;

Kim, Heon Seok ;

Kim, Jin-Soo .

NATURE METHODS, 2014, 11 (07) :705-706

[2] Recent Advances in Genome Editing Using CRISPR/Cas9 [J].

Ding, Yuduan ;

Li, Hong ;

Chen, Ling-Ling ;

Xie, Kabin .

FRONTIERS IN PLANT SCIENCE, 2016, 7

[3] Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 [J].

Doench, John G. ;

Fusi, Nicolo ;

Sullender, Meagan ;

Hegde, Mudra ;

Vaimberg, Emma W. ;

Donovan, Katherine F. ;

Smith, Ian ;

Tothova, Zuzana ;

Wilen, Craig ;

Orchard, Robert ;

Virgin, Herbert W. ;

Listgarten, Jennifer ;

Root, David E. .

NATURE BIOTECHNOLOGY, 2016, 34 (02) :184-+

[4] Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation [J].

Doench, John G. ;

Hartenian, Ella ;

Graham, Daniel B. ;

Tothova, Zuzana ;

Hegde, Mudra ;

Smith, Ian ;

Sullender, Meagan ;

Ebert, Benjamin L. ;

Xavier, Ramnik J. ;

Root, David E. .

NATURE BIOTECHNOLOGY, 2014, 32 (12) :1262-U130

[5] Improving CRISPR-Cas nuclease specificity using truncated guide RNAs [J].

Fu, Yanfang ;

Sander, Jeffry D. ;

Reyon, Deepak ;

Cascio, Vincent M. ;

Joung, J. Keith .

NATURE BIOTECHNOLOGY, 2014, 32 (03) :279-284

[6] Deciphering, Communicating, and Engineering the CRISPR PAM [J].

Leenay, Ryan T. ;

Beisel, Chase L. .

JOURNAL OF MOLECULAR BIOLOGY, 2017, 429 (02) :177-191

[7] Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems [J].

Leenay, Ryan T. ;

Maksimchuk, Kenneth R. ;

Slotkowski, Rebecca A. ;

Agrawal, Roma N. ;

Gomaa, Ahmed A. ;

Briner, Alexandra E. ;

Barrangou, Rodolphe ;

Beisel, Chase L. .

MOLECULAR CELL, 2016, 62 (01) :137-147

[8] CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants [J].

Lei, Yang ;

Lu, Li ;

Liu, Hai-Yang ;

Li, Sen ;

Xing, Feng ;

Chen, Ling-Ling .

MOLECULAR PLANT, 2014, 7 (09) :1494-1496

[9] Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing [J].

Liang, Gang ;

Zhang, Huimin ;

Lou, Dengji ;

Yu, Diqiu .

SCIENTIFIC REPORTS, 2016, 6

[10] SHAPE directed RNA folding [J].

Lorenz, Ronny ;

Luntzer, Dominik ;

Hofacker, Ivo L. ;

Stadler, Peter F. ;

Wolfinger, Michael T. .

BIOINFORMATICS, 2016, 32 (01) :145-147

← 1 2 →