Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp pekinensis)

被引：52

作者：

Zhang, FL

Takahata, Y ^{[1
]}

Watanabe, M

Xu, JB

机构：

[1] Iwate Univ, Fac Agr, Morioka, Iwate 0208550, Japan

[2] Beijing Vegetable Res Ctr, Beijing 100089, Peoples R China

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 06期

关键词：

Chinese cabbage (Brassica campestris L; ssp pekinensis); genetic transformation; Agrobacterium tumefaciens; cotyledonary explant;

D O I：

10.1007/s002990050775

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 min and co-cultivated for 3 days in co-cultivation medium at pH 5.2 supplemented with 10 mg/l acetosyringone. Transgenic plants of all three cultivars used were obtained with frequencies of 1.6-2.7% when the explants were regenerated in shoot regeneration medium solidified with 1.6% agar. A histochemical GUS assay and PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progeny showed that the transgenes were inherited in a Mendelian fashion.

引用

页码：569 / 575

页数：7

共 20 条

[1] Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata [J].