Enhanced immune responses after DNA vaccination with combined envelope genes from different HIV-1 subtypes

被引：56

作者：

Ljungberg, K ^{[1
]}

Rollman, E

Eriksson, L

Hinkula, J

Wahren, B

机构：

[1] Swedish Inst Infect Dis Control, Dept Virol, SE-17182 Solna, Sweden

[2] Karolinska Inst, Ctr Microbiol & Tumor Biol, SE-17182 Stockholm, Sweden

来源：

VIROLOGY | 2002年 / 302卷 / 01期

关键词：

HIV-1; gp160; DNA vaccine;

D O I：

10.1006/viro.2002.1547

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In a multisubtype approach to HIV-1 vaccination, mice were immunized with HIV-1 envelope gp160 genes from subtypes A, B, and C. Subsequently the mice were challenged with syngeneic primary splenocytes infected with a HIV-1/MuLV pseudovirus carrying a subtype B genome. HIV-specific immune responses and protection were strongest in the group of animals immunized with a combination of subtype A, B, and C specific gp160 genes as compared to subtype B only. Immunization with the combination of the cross-reactive subtypes A and C envelope genes induced HIV-specific immune responses but did not result in significant protection to challenge with subtype B infected cells. From this we conclude that immunization with the envelope genes from several HIV-1 subtypes may indeed enhance immune responses. This study shows that by using a mix of subtype envelope genes, an enhanced protective immunity can be obtained experimentally, potentially also in humans. (C) 2002 Elsevier Science (USA).

引用

页码：44 / 57

页数：14

共 50 条

[1] EPITOPE MAPPING AND TOPOLOGY OF BACULOVIRUS-EXPRESSED HIV-1 GP160 DETERMINED WITH A PANEL OF MURINE MONOCLONAL-ANTIBODIES [J].

ABACIOGLU, YH ;

FOUTS, TR ;

LAMAN, JD ;

CLAASSEN, E ;

PINCUS, SH ;

MOORE, JP ;

ROBY, CA ;

KAMINLEWIS, R ;

LEWIS, GK .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1994, 10 (04) :371-381

[2] NEUTRALIZING CROSS-REACTIVE AND NONNEUTRALIZING MONOCLONAL-ANTIBODIES TO HIV-1 GP120 [J].

AKERBLOM, L ;

HINKULA, J ;

BROLIDEN, PA ;

MAKITALO, B ;

FRIDBERGER, T ;

ROSEN, J ;

VILLACRESERIKSSON, M ;

MOREIN, B ;

WAHREN, B .

AIDS, 1990, 4 (10) :953-960

[3] Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine [J].

Amara, RR ;

Villinger, F ;

Altman, JD ;

Lydy, SL ;

O'Neil, SP ;

Staprans, SI ;

Montefiori, DC ;

Xu, Y ;

Herndon, JG ;

Wyatt, LS ;

Candido, MA ;

Kozyr, NL ;

Earl, PL ;

Smith, JM ;

Ma, HL ;

Grimm, BD ;

Hulsey, ML ;

Miller, J ;

McClure, HM ;

McNicholl, JM ;

Moss, B ;

Robinson, HL .

SCIENCE, 2001, 292 (5514) :69-74

[4] Dose-response resistance to HIV-1/MuLV pseudotype virus ex vivo in a hairpin ribozyme transgenic mouse model [J].

Andäng, M ;

Hinkula, J ;

Hotchkiss, G ;

Larsson, S ;

Britton, S ;

Wong-Staal, F ;

Wahren, B ;

Ährlund-Richter, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12749-12753

[5]

[Anonymous], AIDS EP UPD

[6]

Attanasio R, 1997, FEMS IMMUNOL MED MIC, V17, P207, DOI 10.1111/j.1574-695X.1997.tb01014.x

[7] Elicitation of high-frequency cytotoxic T-lymphocyte responses against both dominant and subdominant simian-human immunodeficiency virus epitopes by DNA vaccination of rhesus monkeys [J].

Barouch, DH ;

Craiu, A ;

Santra, S ;

Egan, MA ;

Schmitz, JE ;

Kuroda, MJ ;

Fu, TM ;

Nam, JH ;

Wyatt, LS ;

Lifton, MA ;

Krivulka, GR ;

Nickerson, CE ;

Lord, CI ;

Moss, B ;

Lewis, MG ;

Hirsch, VM ;

Shiver, JW ;

Letvin, NL .

JOURNAL OF VIROLOGY, 2001, 75 (05) :2462-2467

[8] Development of bivalent (B/E) vaccines able to neutralize CCR5-dependent viruses from the United States and Thailand [J].

Berman, PW ;

Huang, W ;

Riddle, L ;

Gray, AM ;

Wrin, T ;

Vennari, J ;

Johnson, A ;

Klaussen, M ;

Prashad, H ;

Köhne, C ;

DeWit, C ;

Gregory, TJ .

VIROLOGY, 1999, 265 (01) :1-9

[9] RAPID CLONING BY HOMOLOGOUS RECOMBINATION IN-VIVO [J].

BUBECK, P ;

WINKLER, M ;

BAUTSCH, W .

NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3601-3602

[10]

Calarota SA, 1999, J IMMUNOL, V163, P2330

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