Enhanced immune responses after DNA vaccination with combined envelope genes from different HIV-1 subtypes

被引:56
作者
Ljungberg, K [1 ]
Rollman, E
Eriksson, L
Hinkula, J
Wahren, B
机构
[1] Swedish Inst Infect Dis Control, Dept Virol, SE-17182 Solna, Sweden
[2] Karolinska Inst, Ctr Microbiol & Tumor Biol, SE-17182 Stockholm, Sweden
关键词
HIV-1; gp160; DNA vaccine;
D O I
10.1006/viro.2002.1547
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In a multisubtype approach to HIV-1 vaccination, mice were immunized with HIV-1 envelope gp160 genes from subtypes A, B, and C. Subsequently the mice were challenged with syngeneic primary splenocytes infected with a HIV-1/MuLV pseudovirus carrying a subtype B genome. HIV-specific immune responses and protection were strongest in the group of animals immunized with a combination of subtype A, B, and C specific gp160 genes as compared to subtype B only. Immunization with the combination of the cross-reactive subtypes A and C envelope genes induced HIV-specific immune responses but did not result in significant protection to challenge with subtype B infected cells. From this we conclude that immunization with the envelope genes from several HIV-1 subtypes may indeed enhance immune responses. This study shows that by using a mix of subtype envelope genes, an enhanced protective immunity can be obtained experimentally, potentially also in humans. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:44 / 57
页数:14
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