Expression of Bcl-2 and PCNA in duct cells after pancreatic duct ligation in rats

被引：30

作者：

Wada, M ^{[1
]}

Doi, R ^{[1
]}

Hosotani, R ^{[1
]}

Lee, JU ^{[1
]}

Fujimoto, K ^{[1
]}

Koshiba, T ^{[1
]}

Miyamoto, Y ^{[1
]}

Fukuoka, S ^{[1
]}

Imamura, M ^{[1
]}

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,SAKYO KU,UJI,KYOTO 60601,JAPAN

来源：

PANCREAS | 1997年 / 15卷 / 02期

关键词：

apoptosis; immunochemistry; Western blot; DNA fragmentation; chronic obstructive pancreatitis;

D O I：

10.1097/00006676-199708000-00010

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Obstruction of the pancreatic duct induces acinar cell deletion followed by duct proliferation and interstitial fibrosis. Apoptosis has been reported to be involved in the induction of acinar cell deletion after pancreatic duct ligation (PDL) in rats, however, the mechanism of pancreatic duct cell proliferation is still unknown. We hypothesized that Bcl-2 (antiapoptosis protein) and PCNA (cell cycle-related protein) could be involved in the mechanism of pancreatic duct cell proliferation after PDL. In PDL rats, acinar cells decreased in number and disappeared completely after duct ligation and duct-lining cells increased in number and formed duct-tubular complexes. Immunohistochemical study showed that PCNA expression appeared in the ductules and centroacinar cells from early stages after duct ligation and that Bcl-2 expression in duct cells? which was faint in normal pancreas, increased significantly when acinar cells were diminishing. Western blotting demonstrated that Bcl-2 was detected as a single band at 26 kDa, and the intensity of Bcl-2 in PDL rats was approximately ninefold stronger than in normal pancreas. Expression of Bcl-2 and PCNA after pancreatic duct ligation may be related to the prevention of apoptosis and cell proliferation of pancreatic duct cells in rats.

引用

页码：176 / 182

页数：7

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