Fixation of conserved sequences shapes human intron size and influences transposon-insertion dynamics

被引：28

作者：

Sironi, M

Menozzi, G

Comi, GP

Bresolin, N

Cagliani, R

Pozzoli, U

机构：

[1] IRCCS, Inst Sci, I-23842 Bosisio Parini, LC, Italy

[2] Univ Milan, IRCCS, Osped Maggiore Policlin, Dipartimento Sci Neurol,Ctr Dino Ferrari, I-20100 Milan, Italy

来源：

TRENDS IN GENETICS | 2005年 / 21卷 / 09期

关键词：

D O I：

10.1016/j.tig.2005.06.009

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The basis for intron expansion in humans is largely unexplored. In this article, we demonstrate that intron expansion has primarily been determined by fixation of multispecies conserved sequences (MCSs) over time. The presence of MCSs has shaped intron features: the insertion of transposable elements (TEs) has been constrained as more MCSs were fixed. Analysis of TE and MCS distribution suggested an unprecedented estimate of information requirements for proper splicing of long introns with indication of sequence constraints extending up to >3 kb downstream 5' splice sites.

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收藏

页码：484 / 488

页数：5

相关论文

共 19 条

[1] Influence of intron length on alternative splicing of CD44 [J].

Bell, MV ;

Cowper, AE ;

Lefranc, MP ;

Bell, JI ;

Screaton, GR .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (10) :5930-5941

[2] Intervening sequences in paralogous genes: A comparative genomic approach to study the evolution of X chromosome introns [J].

Cardazzo, B ;

Bargelloni, L ;

Toffolatti, L ;

Patarnello, T .

MOLECULAR BIOLOGY AND EVOLUTION, 2003, 20 (12) :2034-2041

[3] Selection for short introns in highly expressed genes [J].

Castillo-Davis, CI ;

Mekhedov, SL ;

Hartl, DL ;

Koonin, EV ;

Kondrashov, FA .

NATURE GENETICS, 2002, 31 (04) :415-418

[4] STATISTICAL-ANALYSIS OF VERTEBRATE SEQUENCES REVEALS THAT LONG GENES ARE SCARCE IN GC-RICH ISOCHORES [J].

DURET, L ;

MOUCHIROUD, D ;

GAUTIER, C .

JOURNAL OF MOLECULAR EVOLUTION, 1995, 40 (03) :308-317

[5] Distribution and characterization of regulatory elements in the human genome [J].

Majewski, J ;

Ott, J .

GENOME RESEARCH, 2002, 12 (12) :1827-1836

[6] Non-coding RNAs: the architects of eukaryotic complexity [J].

Mattick, JS .

EMBO REPORTS, 2001, 2 (11) :986-991

[7] G triplets located throughout a class of small vertebrate introns enforce intron borders and regulate splice site selection [J].

McCullough, AJ ;

Berget, SM .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (08) :4562-4571

[8] The evolution of an intron: Analysis of a long, deletion-prone intron in the human dystrophin gene [J].

McNaughton, JC ;

Hughes, G ;

Jones, WA ;

Stockwell, PA ;

Klamut, HJ ;

Petersen, GB .

GENOMICS, 1997, 40 (02) :294-304

[9] Mutational equilibrium model of genome size evolution [J].

Petrov, DA .

THEORETICAL POPULATION BIOLOGY, 2002, 61 (04) :531-544

[10] Comparative analysis of vertebrate dystrophin loci indicate intron gigantism as a common feature [J].

Pozzoli, U ;

Elgar, G ;

Cagliani, R ;

Riva, L ;

Comi, GP ;

Bresolin, N ;

Bardoni, A ;

Sironi, M .

GENOME RESEARCH, 2003, 13 (05) :764-772