Evidence that a starfish egg Src family tyrosine kinase associates with PLC-γ1 SH2 domains at fertilization

被引:56
作者
Giusti, AF [1 ]
Carroll, DJ
Abassi, YA
Foltz, KR
机构
[1] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
[2] Univ Calif Santa Barbara, Inst Marine Sci, Santa Barbara, CA 93106 USA
关键词
PLC-gamma; Src kinase; starfish fertilization;
D O I
10.1006/dbio.1998.9187
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The initiation of calcium release at fertilization in the eggs of most animals relies on the production of IP3, implicating the activation of phospholipase C, Recent work has demonstrated that injection of PLC-gamma SH2 domain fusion proteins into starfish eggs specifically inhibits the initiation of calcium release in response to sperm, indicating that PLC-gamma is necessary for Ca2+ release at fertilization [Carroll et al. (1997) J. Cell Biol. 138, 1303-1311]. Here we investigate how PLC-gamma may be activated, by using the PLC-gamma SH2 domain fusion protein as an affinity matrix to identify interacting proteins. A tyrosine kinase activity and an egg protein of ca. M-r 58 K that is recognized by an antibody directed against Src family tyrosine kinases associate with PLC-gamma SH2 domains in a fertilization-dependent manner. These associations are detected by 15 s postfertilization, consistent with a function in releasing Ca2+. Calcium ionophore treatment of eggs did not cause association of the kinase activity or of the Src family protein with the PLC-gamma SH2 domains. These data identify an egg Src family tyrosine kinase as a potential upstream regulator of PLC-gamma in the activation of starfish eggs. (C) 1999 Academic Press.
引用
收藏
页码:189 / 199
页数:11
相关论文
共 65 条
[1]  
Adachi M, 1996, CELL, V85, P15
[2]  
BOULTON TG, 1994, J BIOL CHEM, V269, P11648
[3]   Regulation, substrates and functions of src [J].
Brown, MT ;
Cooper, JA .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1996, 1287 (2-3) :121-149
[4]   Calcium release at fertilization in starfish eggs is mediated by phospholipase C gamma [J].
Carroll, DJ ;
Ramarao, CS ;
Mehlmann, LM ;
Roche, S ;
Terasaki, M ;
Jaffe, LA .
JOURNAL OF CELL BIOLOGY, 1997, 138 (06) :1303-1311
[5]   Identification of PLCγ-dependent and -independent events during fertilization of sea urchin eggs [J].
Carroll, DJ ;
Albay, DT ;
Terasaki, M ;
Jaffe, LA ;
Foltz, KR .
DEVELOPMENTAL BIOLOGY, 1999, 206 (02) :232-247
[6]   PHOSPHORYLATION OF SYNTHETIC PEPTIDES BY A TYROSINE PROTEIN-KINASE FROM THE PARTICULATE FRACTION OF A LYMPHOMA CELL-LINE [J].
CASNELLIE, JE ;
HARRISON, ML ;
PIKE, LJ ;
HELLSTROM, KE ;
KREBS, EG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :282-286
[7]  
CHENG HC, 1992, J BIOL CHEM, V267, P9248
[8]   2 PHASES OF INOSITOL POLYPHOSPHATE AND DIACYLGLYCEROL PRODUCTION AT FERTILIZATION [J].
CIAPA, B ;
WHITAKER, M .
FEBS LETTERS, 1986, 195 (1-2) :347-351
[9]   A RAPID CHANGE IN PHOSPHORYLATION ON TYROSINE ACCOMPANIES FERTILIZATION OF SEA-URCHIN EGGS [J].
CIAPA, B ;
EPEL, D .
FEBS LETTERS, 1991, 295 (1-3) :167-170
[10]   INJECTION OF A SOLUBLE SPERM FRACTION INTO SEA-URCHIN EGGS TRIGGERS THE CORTICAL REACTION [J].
DALE, B ;
DEFELICE, LJ ;
EHRENSTEIN, G .
EXPERIENTIA, 1985, 41 (08) :1068-1070