Stimulation of tryptophan hydroxylase production in a serotonin-producing cell line (RBL2H3) by intracellular calcium mobilizing reagents

被引：9

作者：

Hasegawa, H ^{[1
]}

Kojima, M ^{[1
]}

Iida, Y ^{[1
]}

Oguro, K ^{[1
]}

Nakanishi, N ^{[1
]}

机构：

[1] MEIKAI UNIV, SCH DENT, DEPT BIOCHEM, SAKADO, SAITAMA 35002, JAPAN

来源：

FEBS LETTERS | 1996年 / 392卷 / 03期

关键词：

tryptophan hydroxylase; serotonin biosynthesis; mast cell; elevation; Ca2+-dependent; A23187; IgE;

D O I：

10.1016/0014-5793(96)00834-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

RBL2H3 cells shelved a remarkable increase in their level of tryptophan hydroxylase (up to 25-fold), the rate-limiting enzyme in serotonin biosynthesis, by stimulation with intracellular calcium mobilizers A23187, thapsigargin, and tBuBHQ as well as by stimulation with an antigen in the presence of IgE, The increase in the enzyme protein was visualized by Western blot analysis using anti-tryptophan hydroxylase antiserum, The enzyme turnover (Hasegawa ct al., FEES Lett,, 368 (1995) 151-154) was not slowed down during the rise in tryptophan hydroxylase. Actinomycin D prevented the stimulation-induced elevation of the enzyme, These findings strongly suggest that this stimulation was achieved by the accelerated biosynthesis of tryptophan hydroxylase.

引用

页码：289 / 292

页数：4

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