Differential co-expression of nicotinic acetylcholine receptor alpha 4 and beta 2 subunit genes in various regions of rat brain

被引：14

作者：

Liu, C ^{[1
]}

Nordberg, A ^{[1
]}

Zhang, X ^{[1
]}

机构：

[1] HUDDINGE UNIV HOSP,KAROLINSKA INST,DIV NICOTINE RES,DEPT CLIN NEUROSCI & FAMILY MED,S-14186 HUDDINGE,SWEDEN

来源：

NEUROREPORT | 1996年 / 7卷 / 10期

关键词：

nicotinic ACh receptor; nicotinic high affinity binding sites; subunit gene transcript; ribonuclease protection assay; brain region; rat;

D O I：

10.1097/00001756-199607080-00024

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE predominant nicotinic acetylcholine receptor (nAChR) subtype in brain binds nicotine with high affinity and has an alpha 4:beta 2 subunit stoichiometry of 2:3. In this study, the mRNA levels of nAChR alpha 4 and beta 2 subunits were simultaneously quantitated by ribonuclease protection assay and compared with the number of alpha 4 beta 2 receptor subtype, measured by (-)-[H-3]nicotine binding in the cerebellum, brain stem, hippocampus, cortex, thalamus and striatum of rat brain. The rank order of abundance of alpha 4 and beta 2 mRNA was different from that of alpha 4 beta 2 receptor subtype levels in the six brain regions studied. The ratio of alpha 4:beta 2 transcripts varied from 2:2.6 to 2:33 in these brain regions. These results reveal a differential co-expression of alpha 4 and beta 2 subunit genes in various regions of rat brain.

引用

页码：1645 / 1649

页数：5

共 21 条

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