Endothelin-induced changes in intracellular calcium in human microglia

被引：23

作者：

McLarnon, JG

Wang, X

Bae, JH

Kim, SU

机构：

[1] Univ British Columbia, Fac Med, Dept Pharmacol & Therapeut, Vancouver, BC V6T 1Z3, Canada

[2] Univ British Columbia, Fac Med, Dept Med, Div Neurol, Vancouver, BC V6T 2B5, Canada

来源：

NEUROSCIENCE LETTERS | 1999年 / 263卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

human microglia; intracellular calcium; calcium influx; endothelin; ETB receptors;

D O I：

10.1016/S0304-3940(99)00082-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Calcium-sensitive spectrofluorometry was used to study the effects of endothelin on levels of intracellular calcium [Ca2+](i) in cultured human microglia. Both ET-I and ET-3 induced transient, non-desensitizing, increases in [Ca2+](i) in over 80% of the cells studied. The responses to either ET-1 or ET-3 were significantly diminished in amplitude and duration in Ca2+-free solution suggesting a prominent contribution of Ca2+ influx to the response. ET-1 induced changes in [Ca2+](i) were not altered in the presence of the selective ETA antagonist BQ610 but were significantly reduced with the selective ETB antagonist BQ780. These results confirm the expression of ETB receptors on human microglia, these receptors may serve a role in a signaling pathway between microglia and endothelial cells. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：9 / 12

页数：4

共 13 条

[1] ENDOTHELIN INDUCES A SUSTAINED RISE IN INTRACELLULAR CALCIUM IN HIPPOCAMPAL ASTROCYTES [J].