Rapid screening of natural products by high-resolution high-temperature gas chromatography

被引：9

作者：

Carbonell, SA

Neto, FRD

Cardoso, JN

Pereira, AD

Amaral, ACF

Barnes, RA

机构：

[1] Univ Fed Rio de Janeiro, LADETEC, Inst Quim, BR-21949900 Rio De Janeiro, Brazil

[2] Lab Quim Prod Nat, BR-21041250 Far Manguinhos, RJ, Brazil

[3] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, BR-21941590 Rio De Janeiro, Brazil

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2000年 / 38卷 / 06期

关键词：

D O I：

10.1093/chromsci/38.6.234

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.

引用

页码：234 / 240

页数：7

共 38 条

[1]

AMARAL ACF, 1996, THESIS U FEDERAL RIO

[2]

THE ALKALOIDS OF CROTON-SALUTARIS [J].