Fast HPLC with non-porous packing materials in the pharmaceutical industry

被引：5

作者：

Wieser, M

Malz, P

Arendt, T

Küppers, S

机构：

[1] Schering AG, In Proc Control, D-13342 Berlin, Germany

[2] Humboldt Univ, D-10117 Berlin, Germany

来源：

CHROMATOGRAPHIA | 2000年 / 51卷 / 7-8期

关键词：

column liquid chromatography; fast analysis; non-porous packing materials; method-transfer;

D O I：

10.1007/BF02490488

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This payer demonstrates the possibilities in transfering HPLC methods from porous to non-porous stationary phases. The procedures are transferred from 125 or 250 mm columns packed with porous stationary phases to 33 mm columns packed with non-porous particles. It is demonstrated that fast HPLC using non-porous columns reduces analysis times by a factor four to eight. Precision is comparable to HPLC with porous stationary phases. The costs for HPLC with porous and with nonporous packing materials are similar. The implementation of those fast HPLC columns is easy because standard equipment can be used.

引用

页码：478 / 482

页数：5

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