A cDNA clone for β-caryophyllene synthase from Artemisia annua

被引：116

作者：

Cai, Y

Jia, JW

Crock, J

Lin, ZX

Chen, XY

Croteau, R ^{[1
]}

机构：

[1] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[3] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Shanghai 200030, Peoples R China

来源：

PHYTOCHEMISTRY | 2002年 / 61卷 / 05期

关键词：

Artemisia annua; Asteraceae; sweet wormwood; homology-based cDNA cloning; recombinant protein expression; sesquiterpene synthase; beta-caryophyllene synthase; sesquiterpene; beta-caryophyllene; gene expression; elicitation;

D O I：

10.1016/S0031-9422(02)00265-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to beta-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, beta-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The beta-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for beta-caryophyllene in plant defense. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：523 / 529

页数：7

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