High-pressure effects on lysosome integrity and lysosomal enzyme activity in bovine muscle

被引:23
作者
Jung, S
de Lamballerie-Anton, M
Taylor, RG
Ghoul, M
机构
[1] ENITIAA, Dept Genie Procedes Alimentaires, F-44322 Nantes 3, France
[2] INRA, Rech Viande Stn, F-63122 St Genes Champanelle, France
[3] ENSAIA, F-54500 Vandoeuvre Les Nancy, France
关键词
high pressure; meat; bovine muscle; lysosome; cathepsin;
D O I
10.1021/jf9908648
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
This study was conducted to determine whether the application of high hydrostatic pressure could modify the enzymatic activity and membrane integrity of lysosomes in muscle. Several combinations of pressure (0-600 MPa) and time (0-300 s) were applied to two types of samples: purified enzymes (cathepsin D and acid phosphatase) in buffer solution and intact muscle (biceps femoris). The enzymes studied showed varying degrees of susceptibility depending on the level of pressure, holding time, and environment. Acid phosphatase activity was minimally affected by pressure in buffer solution, whereas cathepsin D was modulated significantly by-the pressure and time applied. The activities of the enzymes extracted from meat increased with pressure. The cytochemical observations showed the presence of primary and secondary lysosomes in muscles. After pressurization, the membrane integrity of the lysosomes was modified. A correlation could be established between lysosomal enzymatic activities and the lysosome membrane breakdown.
引用
收藏
页码:2467 / 2471
页数:5
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