Recognition of aqueous flavin mononucleotide on the surface of binary monolayers of guanidinium and melamine amphiphiles

被引:60
作者
Ariga, K [1 ]
Kamino, A [1 ]
Koyano, H [1 ]
Kunitake, T [1 ]
机构
[1] JST,SUPERMOL PROJECT,KURUME,FUKUOKA 839,JAPAN
关键词
D O I
10.1039/a700081b
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Recognition of aqueous flavin mononucleotide (FMN) on the surface of binary monolayers of guanidinium amphiphiles (monoalkyl derivative, C(18)Gua, or dialkyl derivative, 2C(18)Gua) and the melamine amphiphile (2C(18)mela-NN) has been investigated by pi-A isotherms, FTIR spectroscopy, and XPS measurements. pi-A Isotherms and FTIR spectra of C(18)Gua-2C(18)mela-NN(1:1) monolayers show that there is no direct hydrogen bonding and/or coulombic interactions between C(18)Gua and 2C(18)mela-NN on pure water and that the C(18)Gua component is dissolved into the subphase upon compression. In contrast, the presence of aqueous FMN prevented C(18)Gua molecules from dissolving into the subphase. Maximum condensation was observed at a 1:1 ratio in C(18)Gua-2C(18)mela-NN mixed monolayers on aqueous FMN. XPS analyses revealed that one FMN molecule was bound to one C(18)Gua-2C(18)mela-NN(1:1) unit and binding was saturated at 5 x 10(-6) mol dm(-3) FMN. Peak shifts observed in FTIR spectra indicated that the isoalloxiazine ring in FMN formed hydrogen bonds with 2C(18)mela-NN. These results support a model that the isoalloxazine and phosphate functions in FMN are bound via hydrogen bonding to melamine in 2C(18)mela-NN and guanidinium in C(18)Gua, respectively. Similar binding behaviour of FMN was observed for mixed monolayers of 2C(18)Gua-2C(18)mela-NN.
引用
收藏
页码:1155 / 1161
页数:7
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