Translation termination in pyrrolysine-utilizing archaea

被引：21

作者：

Alkalaeva, Elena ^{[1
]}

Eliseev, Boris ^{[1
]}

Ambrogelly, Alexandre ^{[3
]}

Vlasov, Peter ^{[1
]}

Kondrashov, Fyodor A. ^{[2
]}

Gundllapalli, Sharath ^{[3
]}

Frolova, Lyudmila ^{[1
]}

Soll, Dieter ^{[3
,4
]}

Kisselev, Lev ^{[1
]}

机构：

[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 119991, Russia

[2] Ctr Genom Regulat, Bioinformat & Genom Programme, Barcelona 08003, Spain

[3] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[4] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

FEBS LETTERS | 2009年 / 583卷 / 21期

基金：

俄罗斯基础研究基金会; 美国国家科学基金会;

关键词：

Translation termination; Archeon; Pyrrolysine-utilizing archea; aRF1; Polypeptide release factor; TRANSFER-RNA SYNTHETASE; RELEASE FACTOR ERF1; STOP CODON; IN-VITRO; AMINO-ACIDS; SELENOCYSTEINE; RECOGNITION; UAG; DOMAIN; MODES;

D O I：

10.1016/j.febslet.2009.09.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although some data link archaeal and eukaryotic translation, the overall mechanism of protein synthesis in archaea remains largely obscure. Both archaeal (aRF1) and eukaryotic (eRF1) single release factors recognize all three stop codons. The archaeal genus Methanosarcinaceae contains two aRF1 homologs, and also uses the UAG stop to encode the 22nd amino acid, pyrrolysine. Here we provide an analysis of the last stage of archaeal translation in pyrrolysine-utilizing species. We demonstrated that only one of two Methanosarcina barkeri aRF1 homologs possesses activity and recognizes all three stop codons. The second aRF1 homolog may have another unknown function. The mechanism of pyrrolysine incorporation in the Methanosarcinaceae is discussed. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3455 / 3460

页数：6

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