Coxiella burnetii plasmid types QpDG and QpH1 are closely related and likely identical

被引:21
作者
Jäger, C [1 ]
Lautenschläger, S [1 ]
Willems, H [1 ]
Baljer, G [1 ]
机构
[1] Univ Giessen, Inst Hyg & Infect Dis Anim, D-35392 Giessen, Germany
关键词
Coxiella burnetii; plasmid; QpDG; PFGE; XL PCR;
D O I
10.1016/S0378-1135(02)00155-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Strains and isolates of Coxiella burnetii, an obligate intracellular bacterium, carry a single plasmid or a plasmid-homologous sequence integrated into the chromosome. The plasmids QpH1, QpRS, QpDV and the chromosome-integrated plasmid-homologous region have been completely sequenced, whereas no sequence data are available for the QpDG plasmid. In this study, we used total genomic DNA from reference strain C burnetii Dugway 5J108-111 to demonstrate and characterize the QpDG plasmid by pulsed-field gel electrophoresis (PFGE) and Southern hybridization. Primers derived from regions shared among C burnetii plasmids were used to construct a physical map of the QpDG plasmid by extra long (XL) PCR. Both approaches, Southern hybridization and XL PCR indicated that QpDG and QpH1 represent a closely related and likely identical plasmid. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:161 / 166
页数:6
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