Involvement of a chromatin remodeling complex in damage tolerance during DNA replication

被引：78

作者：

Falbo, Karina B. ^{[1
]}

Alabert, Constance ^{[2
]}

Katou, Yuki ^{[3
]}

Wu, Su ^{[4
]}

Han, Junhong ^{[5
]}

Wehr, Tammy ^{[1
]}

Xiao, Jing ^{[1
]}

He, Xiangwei ^{[6
]}

Zhang, Zhiguo ^{[5
]}

Shi, Yang ^{[4
]}

Shirahige, Katsu ^{[3
]}

Pasero, Philippe ^{[2
]}

Shen, Xuetong ^{[1
]}

机构：

[1] MD Anderson Canc Ctr, Div Sci Pk Res, Dept Carcinogenesis, Smithville, TX USA

[2] CNRS, Inst Human Genet, UPR 1142, Montpellier, France

[3] Tokyo Inst Technol, Ctr Gene Res, Midori Ku, Yokohama, Kanagawa 227, Japan

[4] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[5] Mayo Clin, Coll Med, Dept Biochem, Rochester, MN USA

[6] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 11期

基金：

美国国家卫生研究院;

关键词：

SACCHAROMYCES-CEREVISIAE; HOMOLOGOUS RECOMBINATION; FORK PROGRESSION; CELL-CYCLE; REPAIR; INO80; PHOSPHORYLATION; SUMOYLATION; UBIQUITIN; PROTEINS;

D O I：

10.1038/nsmb.1686

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling complex in the DNA damage tolerance pathways. Whereas INO80 is necessary for the resumption of replication at forks stalled by methyl methane sulfonate (MMS), it is not required for replication fork collapse after treatment with hydroxyurea (HU). Mechanistically, INO80 regulates DNA damage tolerance during replication through modulation of PCNA (proliferating cell nuclear antigen) ubiquitination and Rad51-mediated processing of recombination intermediates at impeded replication forks. Our findings establish a mechanistic link between INO80 and DNA damage tolerance pathways, indicating that chromatin remodeling is important for accurate DNA replication.

引用

页码：1167 / U7

页数：7

共 36 条

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