Enzymatic recognition of acyclic universal base analogues in oligonucleotides

被引：10

作者：

Loakes, D ^{[1
]}

VanAerschot, A ^{[1
]}

Brown, DM ^{[1
]}

Hill, F ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,REGA INST,MED CHEM LAB,B-3000 LOUVAIN,BELGIUM

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 11-12期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1080/07328319608002740

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the acyclic analogues 3 and 4 on recognition by commonly used enzymes in recombinant DNA work have been investigated. This report shows that acyclic sugars can function in place of the normal 2'-deoxyribose moiety with some enzymes, expanding the range of possible manipulations.

引用

页码：1891 / 1904

页数：14

共 19 条

[1] ISOTACTIC GLYCERO OLIGOTHYMIDYLATE - A CONVENIENT PREPARATION OF (R)-1',2'-SECO-2'-NORTHYMIDINE AND (S)-1',2'-SECO-2'-NORTHYMIDINE [J].

AZYMAH, M ;

CHAVIS, C ;

LUCAS, M ;

MORVAN, F ;

IMBACH, JL .

NUCLEOSIDES & NUCLEOTIDES, 1992, 11 (06) :1241-1255

[2] SYNTHESIS, STRUCTURE, AND DEOXYRIBONUCLEIC-ACID SEQUENCING WITH A UNIVERSAL NUCLEOSIDE - 1-(2'-DEOXY-BETA-D-RIBOFURANOSYL)-3-NITROPYRROLE [J].

BERGSTROM, DE ;

ZHANG, PM ;

TOMA, PH ;

ANDREWS, PC ;

NICHOLS, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (04) :1201-1209

[3] Oligonucleotides containing acyclic nucleoside analogues with carbamate internucleoside linkages [J].

Habus, I ;

Agrawal, S .

NUCLEOSIDES & NUCLEOTIDES, 1995, 14 (9-10) :1853-1859

[4] PRODUCTION OF SINGLE-STRANDED-DNA TEMPLATES BY EXONUCLEASE DIGESTION FOLLOWING THE POLYMERASE CHAIN-REACTION [J].

HIGUCHI, RG ;

OCHMAN, H .

NUCLEIC ACIDS RESEARCH, 1989, 17 (14) :5865-5865

[5] 2'-O-ALKYL OLIGORIBONUCLEOTIDES AS ANTISENSE PROBES [J].

IRIBARREN, AM ;

SPROAT, BS ;

NEUNER, P ;

SULSTON, I ;

RYDER, U ;

LAMOND, AI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7747-7751

[6]

LITTLE JW, 1967, J BIOL CHEM, V242, P679

[7]

LIU H, 1994, BIOTECHNIQUES, V16, P24

[8] 3-NITROPYRROLE AND 5-NITROINDOLE AS UNIVERSAL BASES IN PRIMERS FOR DNA-SEQUENCING AND PCR [J].

LOAKES, D ;

BROWN, DM ;

LINDE, S ;

HILL, F .

NUCLEIC ACIDS RESEARCH, 1995, 23 (13) :2361-2366

[9]

LOAKES D, 1994, NUCL ACIDS RES, V20

[10]

MANIATIS T, MOL CLONING

← 1 2 →