Identification and functional characterization of the Neisseria gonorrhoeae lbpB gene product

被引：40

作者：

Biswas, GD

Anderson, JE

Chen, CJ

Cornelissen, CN

Sparling, PF

机构：

[1] Univ N Carolina, Dept Med, Sch Med, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Microbiol & Immunol, Sch Med, Chapel Hill, NC 27599 USA

[3] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 USA

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 01期

关键词：

D O I：

10.1128/IAI.67.1.455-459.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We cloned lbpB, encoding a predicted 80-kDa lipoprotein, upstream of lbpA. A nonpolar mutant (LbpB(-) LbpA(+)) had normal lactoferrin (LF) binding and grew normally with LF as an iron source, whereas LbpB(-) LbpA(-) and LbpB(+) LbpA(-) strains had reduced binding of LF and did not grow with LF as an iron source. LbpB bound LF directly in an affinity purification, suggesting that LbpB might play a still-uncharacterized role in the LF iron utilization.

引用

收藏

页码：455 / 459

页数：5

相关论文

共 33 条

[1] A GTP-BINDING PROTEIN OF ESCHERICHIA-COLI HAS HOMOLOGY TO YEAST RAS PROTEINS [J].

AHNN, J ;

MARCH, PE ;

TAKIFF, HE ;

INOUYE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (23) :8849-8853

[2] GONOCOCCAL TRANSFERRIN-BINDING PROTEIN-2 FACILITATES BUT IS NOT ESSENTIAL FOR TRANSFERRIN UTILIZATION [J].

ANDERSON, JE ;

SPARLING, PF ;

CORNELISSEN, CN .

JOURNAL OF BACTERIOLOGY, 1994, 176 (11) :3162-3170

[3] CHARACTERIZATION OF LBPA, THE STRUCTURAL GENE FOR A LACTOFERRIN RECEPTOR IN NEISSERIA-GONORRHOEAE [J].

BISWAS, GD ;

SPARLING, PF .

INFECTION AND IMMUNITY, 1995, 63 (08) :2958-2967

[4] LINEARIZATION OF DONOR DNA DURING PLASMID TRANSFORMATION IN NEISSERIA-GONORRHOEAE [J].

BISWAS, GD ;

BURNSTEIN, KL ;

SPARLING, PF .

JOURNAL OF BACTERIOLOGY, 1986, 168 (02) :756-761

[5] GENETIC-EVIDENCE THAT NEISSERIA-GONORRHOEAE PRODUCES SPECIFIC RECEPTORS FOR TRANSFERRIN AND LACTOFERRIN [J].

BLANTON, KJ ;

BISWAS, GD ;

TSAI, J ;

ADAMS, J ;

DYER, DW ;

DAVIS, SM ;

KOCH, GG ;

SEN, PK ;

SPARLING, PF .

JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5225-5235

[6] Preparation and characterization of Neisseria meningitidis mutants deficient in production of the human lactoferrin-binding proteins LbpA and LbpB [J].

Bonnah, RA ;

Schryvers, AB .

JOURNAL OF BACTERIOLOGY, 1998, 180 (12) :3080-3090

[7] Identification and purification of a hemoglobin-binding outer membrane protein from Neisseria gonorrhoeae [J].

Chen, CJ ;

Sparling, PF ;

Lewis, LA ;

Dyer, DW ;

Elkins, C .

INFECTION AND IMMUNITY, 1996, 64 (12) :5008-5014

[8] The transferrin receptor expressed by gonococcal strain FA1090 is required for the experimental infection of human male volunteers [J].

Cornelissen, CN ;

Kelley, M ;

Hobbs, MM ;

Anderson, JE ;

Cannon, JG ;

Cohen, MS ;

Sparling, PF .

MOLECULAR MICROBIOLOGY, 1998, 27 (03) :611-616

[9] Binding and surface exposure characteristics of the gonococcal transferrin receptor are dependent on both transferrin-binding proteins [J].

Cornelissen, CN ;

Sparling, PF .

JOURNAL OF BACTERIOLOGY, 1996, 178 (05) :1437-1444

[10] IRON PIRACY - ACQUISITION OF TRANSFERRIN-BOUND IRON BY BACTERIAL PATHOGENS [J].

CORNELISSEN, CN ;

SPARLING, PF .

MOLECULAR MICROBIOLOGY, 1994, 14 (05) :843-850

← 1 2 3 4 →