Vascular endothelial growth factor-A is a survival factor for nucleus pulposus cells in the intervertebral disc

被引：118

作者：

Fujita, Nobuyuki ^{[1
,2
]}

Imai, Jun-ichi ^{[7
]}

Suzuki, Toru ^{[1
,2
]}

Yamada, Masayuki ^{[6
,8
]}

Ninomiya, Ken ^{[1
,2
]}

Miyamoto, Kana ^{[1
,2
]}

Iwasaki, Ryotaro ^{[2
,5
]}

Morioka, Hideo ^{[1
,4
]}

Matsumoto, Morio ^{[1
]}

Chiba, Kazuhiro ^{[1
,4
]}

Watanabe, Shinya ^{[7
]}

Suda, Toshio ^{[2
]}

Toyama, Yoshiaki ^{[1
]}

Miyamoto, Takeshi ^{[1
,2
,3
,4
,9
]}

机构：

[1] Keio Univ, Sch Med, Dept Orthoped Surg, Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Sch Med, Sakaguchi Lab,Dept Cell Differentiat, Shinjuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Sch Med, Dept Musculoskeletal Reconstruct & Regenerat Surg, Shinjuku Ku, Tokyo 1608582, Japan

[4] Keio Univ, Sch Med, Div Orthoped Res, Shinjuku Ku, Tokyo 1608582, Japan

[5] Keio Univ, Sch Med, Dept Dent & Oral Surg, Shinjuku Ku, Tokyo 1608582, Japan

[6] Keio Univ, Sch Med, Ctr Integrated Med Res, Shinjuku Ku, Tokyo 1608582, Japan

[7] Tokyo Med & Dent Univ, Dept Clin Informat, Bunkyo Ku, Tokyo 1138519, Japan

[8] Cent Inst Expt Anim, Biomed Res Dept, Kawasaki, Kanagawa, Japan

[9] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol, Kawaguchi, Saitama 3320012, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 02期

关键词：

nucleus pulposus; vascular endothelial growth factor-A; intervertebral disc; avascular;

D O I：

10.1016/j.bbrc.2008.05.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The intervertebral disc (IVD) is composed of two avascular tissue types, the nucleus pulposus (NP) and the annulus fibrosus (AF). IVDs is the largest avascular tissue in the human body, however, how these tissues are maintained without a blood supply is poorly understood. Here we show that vascular endothelial growth factor-A (VEGF-A) is highly expressed in NP and that VEGF-A plays a role in NP survival. High VEGF-A expression in NP was detected by microarray analysis, and NP was positive for the hypoxic probe pimonidazole and hypoxia-responsive genes. VEGF-A expression in NP was promoted by hypoxic conditions in vitro. NP cells also expressed the membrane-bound VEGF receptor-1 (VEGFR-1), and the number of apoptotic cells in cultured cell model of NP increased following treatment with VEGFR-1-Fc, which traps VEGF-A in NP. These results indicate that NP is a hypoxic tissue, and that VEGF-A functions in NP survival in an autocrine/paracrine manner. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：367 / 372

页数：6

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