Levels of 8-hydroxy-2′-deoxyguanosine in cellular DNA from 12 tissues of young and old Sprague-Dawley rats

被引:33
作者
Schmerold, I
Niedermüller, H
机构
[1] Vet Univ Vienna, Inst Pharmacol & Toxicol, A-1210 Vienna, Austria
[2] Ludwig Boltzmann Inst Expt Gerontol, A-1210 Vienna, Austria
[3] Vet Univ Vienna, Inst Physiol, A-1210 Vienna, Austria
关键词
oxidative DNA damage; 8-hydroxy-2 '-deoxyguanosine; ageing; rat;
D O I
10.1016/S0531-5565(01)00103-6
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
The age dependent increase of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) reported in DNA of organs of old rats appears to vary with the strain, age and sex of the animals used for the investigation. Here we report on 8-OH-dG concentrations in the cellular DNA of 12 tissues of male Sprague-Dawley rats aged 5 or 30 months and kept under standard conditions throughout their lives until being killed. DNA from frozen organs was isolated using a Qiagen DNA purification kit. Following digestion (nuclease Pl, alkaline nuclease) hydrolysed DNA was applied onto a HPLC column; native nucleosides were monitored at 254 nm and 8-OH-dG by electrochemical detection. 8-OH-dG levels in organs of young rats ranged between 10 and 90 mu mol/mol deoxyguanosine (dG). Highest levels (pmol 8-OH-dG fmol dG) were detected in the oesophagus (90), muscle (61), brain (65), liver (59), spleen (57), and testicles (63). 8-OH-dG in DNA from kidney, lung, heart, small and large intestine ranged between 28 and 38 mu mol/mol dG. Lowest amounts were found in the glandular stomach (10). DNA of old rats generally contained higher 8-OH-dG levels with significant increases in liver (186%) and kidney (372%); other organs showed no significant decreases (spleen, brain, testicles) or increases up to 69% (heart). These findings are discussed in the context with previously published data on 8-OH-dG levels in organs from young and old rats. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:1375 / 1386
页数:12
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