Surface modification of Ti-6Al-4 V alloy for biomineralization and specific biological response: part II, alkaline phosphatase grafting

被引：27

作者：

Ferraris, S. ^{[1
]}

Spriano, S. ^{[1
]}

Bianchi, C. L. ^{[2
]}

Cassinelli, C. ^{[3
]}

Verne, E. ^{[1
]}

机构：

[1] Politecn Torino, DISMIC Dept, I-10129 Turin, Italy

[2] Univ Milan, Phys Chem & Electrochem Dept, I-20133 Milan, Italy

[3] NobilBio Ric, I-14037 Portacomaro, AT, Italy

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2011年 / 22卷 / 08期

关键词：

ALkaline phosphatase - Biological functionalization - Biological response - Biological signals - Research fields - Surface Functionalization - Synergistic effect - Ti-6Al-4V alloy;

D O I：

10.1007/s10856-011-4365-9

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Titanium and its alloys are the most widespread materials for the realization of orthopaedic and dental implants due to their good mechanical properties and biocompatibility. Surface functionalization of biomaterials aimed to improve and quicken implant integration and tissue regeneration is an active research field. The opportunity to confer biological activity (ability to directly stimulate cells with proper biological signals) to the Ti6Al4 V alloy, previously modified to be bioactive from the inorganic point of view (apatite precipitation), was explored in this research work. The alkaline phosphatase (ALP) enzyme was grafted to metal surface via tresyl chloride activation, maintaining its activity. A synergistic effect between biological functionalization and inorganic bioactivity was observed.

引用

页码：1835 / 1842

页数：8

共 24 条

[1]

APPLEBUR.ML, 1970, J BIOL CHEM, V245, P4968

[2]

Alkaline phosphatase-induced mineral deposition to anchor collagen fibrils to a solid surface [J].