Second-harmonic generation scattering directionality predicts tumor cell motility in collagen gels

被引:17
作者
Burke, Kathleen A. [1 ]
Dawes, Ryan P. [2 ]
Cheema, Mehar K. [3 ]
Van Hove, Amy [1 ]
Benoit, Danielle S. W. [1 ,4 ,5 ,6 ]
Perry, Seth W. [1 ]
Brown, Edward [1 ,2 ]
机构
[1] Univ Rochester, Dept Biomed Engn, Rochester, NY 14627 USA
[2] Univ Rochester, Dept Neurobiol & Anat, Rochester, NY 14642 USA
[3] SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11790 USA
[4] Univ Rochester, Dept Biomed Genet, Rochester, NY 14642 USA
[5] Univ Rochester, Dept Chem Engn, Rochester, NY 14627 USA
[6] Univ Rochester, Med Ctr, Ctr Musculoskeletal Res, Rochester, NY 14642 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
second-harmonic generation; multiphoton microscopy; F/B ratio; collagen; metastasis; BREAST-CANCER; MOVEMENT;
D O I
10.1117/1.JBO.20.5.051024
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
Second-harmonic generation (SHG) allows for the analysis of tumor collagen structural changes throughout metastatic progression. SHG directionality, measured through the ratio of the forward-propagating to backward-propagating signal (F/B ratio), is affected by collagen fibril diameter, spacing, and disorder of fibril packing within a fiber. As tumors progress, these parameters evolve, producing concurrent changes in F/B. It has been recently shown that the F/B of highly metastatic invasive ductal carcinoma (IDC) breast tumors is significantly different from less metastatic tumors. This suggests a possible relationship between the microstructure of collagen, as measured by the F/B, and the ability of tumor cells to locomote through that collagen. Utilizing in vitro collagen gels of different F/B ratios, we explored the relationship between collagen microstructure and motility of tumor cells in a "clean" environment, free of the myriad cells, and signals found in in vivo. We found a significant relationship between F/B and the total distance traveled by the tumor cell, as well as both the average and maximum velocities of the cells. Consequently, one possible mechanism underlying the observed relationship between tumor F/B and metastatic output in IDC patient samples is a direct influence of collagen structure on tumor cell motility. (C) The Authors.
引用
收藏
页数:9
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