Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry

被引：329

作者：

Holmes, David ^{[1
,2
]}

Pettigrew, David ^{[3
]}

Reccius, Christian H. ^{[3
]}

Gwyer, James D. ^{[3
]}

van Berkel, Cees ^{[3
]}

Holloway, Judith ^{[2
]}

Davies, Donna E. ^{[2
]}

Morgan, Hywel ^{[1
]}

机构：

[1] Univ Southampton, Sch Elect & Comp Sci, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Sch Med, Div Infect Inflammat & Repair, Southampton SO16 6YD, Hants, England

[3] Philips Res Labs, Cambridge CB4 0FY, England

来源：

LAB ON A CHIP | 2009年 / 9卷 / 20期

基金：

英国工程与自然科学研究理事会;

关键词：

HEMOLYSIS;

D O I：

10.1039/b910053a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system.

引用

页码：2881 / 2889

页数：9

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