Detection of aberrant methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions

被引:83
作者
Huang, Zhao-Hui
Li, Li-Hua
Yang, Fan
Wang, Jin-Fu
机构
[1] Soochow Univ, Oncol Inst Wuxi, Affiliated Hosp 4, Wuxi 214062, Jiangsu, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310012, Peoples R China
关键词
colorectal cancer; methylation; feces; secreted frizzled-related protein gene 2; hyperplastic polyposis protein gene; methylguanine-DNA methyltransferase gene;
D O I
10.3748/wjg.v13.i6.950
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To investigate the feasibility of detecting methylated fecal DNA as a screening tool for colorectal carcinoma (CRC) and precancerous lesions. METHODS: Methylated secreted frizzled-related protein gene 2 (SFRP2), hyperplastic polyposis protein gene (HPP1) and O-6-methylguanine-DNA methyltransferase gene (MGMT) in stools from 52 patients with CRC, 35 patients with benign colorectal diseases and 24 normal individuals were analyzed using methylation-specific PCR. RESULTS: Methylated SFRP2, HPP1 and MGMT were detected in 94.2%, 71.2%, 48.1% of CRC patients and 52.4%, 57.1%, 28.6% of adenoma patients, respectively. The overall prevalence of fecal DNA with at least one methylated gene was 96.2% and 81.8% in patients with CRC and precancerous lesions, respectively. In contrast, only one of the 24 normal individuals revealed methylated DNA. These results indicated a 93.7% sensitivity and a 77.1% specificity of the assay for detecting CRC and precancerous lesions. CONCLUSION: Methylation testing of fecal DNA using a panel of epigenetic markers may be a simple and promising non-invasive screening method for CRC and precancerous lesions. (C) 2007 The WJG Press. All rights reserved.
引用
收藏
页码:950 / 954
页数:5
相关论文
共 19 条
  • [1] Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction?
    Baylin, SB
    Ohm, JE
    [J]. NATURE REVIEWS CANCER, 2006, 6 (02) : 107 - 116
  • [2] Belshaw NJ, 2004, CANCER EPIDEM BIOMAR, V13, P1495
  • [3] CpG island methylation in aberrant crypt foci of the colorectum
    Chan, AOO
    Broaddus, RR
    Houlihan, PS
    Issa, JPJ
    Hamilton, SR
    Rashid, A
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2002, 160 (05) : 1823 - 1830
  • [4] Detection in fecal DNA of colon cancer-specific methylation of the nonexpressed vimentin gene
    Chen, WD
    Han, ZJ
    Skoletsky, J
    Olson, J
    Sah, J
    Myeroff, L
    Platzer, P
    Lu, SL
    Dawson, D
    Willis, J
    Pretlow, TR
    Lutterbaugh, J
    Kasturi, L
    Willson, JKV
    Rao, JS
    Shuber, A
    Markowitz, SD
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2005, 97 (15): : 1124 - 1132
  • [5] Hypermethylation of the TPEF/HPP1 gene in primary and metastatic colorectal cancers
    Ebert, MPA
    Mooney, SH
    Tonnes-Priddy, L
    Lograsso, J
    Hoffmann, J
    Chen, J
    Röcken, C
    Schulz, HU
    Malfertheiner, P
    Lofton-Day, C
    [J]. NEOPLASIA, 2005, 7 (08): : 771 - 778
  • [6] Esteller M, 1999, CANCER RES, V59, P793
  • [7] Hyperplastic-like colon polyps that preceded microsatellite-unstable adenocarcinomas
    Goldstein, NS
    Bhanot, P
    Odish, E
    Hunter, S
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2003, 119 (06) : 778 - 796
  • [8] Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands
    Herman, JG
    Graff, JR
    Myohanen, S
    Nelkin, BD
    Baylin, SB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) : 9821 - 9826
  • [9] Cancer statistics, 2006
    Jemal, A
    Siegel, R
    Ward, E
    Murray, T
    Xu, JQ
    Smigal, C
    Thun, MJ
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2006, 56 (02) : 106 - 130
  • [10] Epigenetic changes in colorectal cancer
    Kondo, Y
    Issa, JPJ
    [J]. CANCER AND METASTASIS REVIEWS, 2004, 23 (1-2) : 29 - 39