NCX1 Na/Ca exchanger splice variants in pancreatic islet cells

被引:29
作者
Van Eylen, F
Bollen, A
Herchuelz, A
机构
[1] Free Univ Brussels, Sch Med, Pharmacol Lab, B-1070 Brussels, Belgium
[2] Free Univ Brussels, Sch Med, Lab Appl Genet, B-1070 Brussels, Belgium
关键词
D O I
10.1677/joe.0.1680517
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the rat pancreatic beta -cell, Na/Ca exchange displays a quite high capacity. The cell is equipped with two alternatively spliced Na/Ca exchanger-1 (NCX1) isoforms, namely NCX1.3 and NCX1.7. To examine the existence of a possible functional difference between these splice variants, they were cloned, together with the heart variant NCX1.1, and expressed in human embryonic kidney-293 (HEK293) and Chinese hamster ovary (CHO) cells. In these two systems, the three splice variants showed a comparable level of intracellular Na+ (Na-i(+))dependent extracellular Ca2+ (Ca-o(2+)) uptake. Different levels of NCX1.3 and NCX1.7 transcripts were found in four rodent species, together with a marked interspecies difference in Na/Ca exchange activity. Three additional splice variants were found (NCX1.2, NCX1.9 and NCX1.13) in guinea-pigs, hamsters and mice, again in different proportions. Our data provide evidence for the activity of the three NCX1 splice variants. They also show the existence of a differential and species-specific transcription pattern of NCX1 gene products in pancreatic islet cells.
引用
收藏
页码:517 / 526
页数:10
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