Mesenchymal Stem Cell-Derived Exosomes: Immunomodulatory Evaluation in an Antigen-Induced Synovitis Porcine Model

被引:74
作者
Casado, Javier G. [1 ,2 ,3 ]
Blazquez, Rebeca [1 ,3 ]
Javier Vela, Francisco [1 ]
Alvarez, Veronica [1 ]
Tarazona, Raquel [2 ]
Miguel Sanchez-Margallo, Francisco [1 ,3 ]
机构
[1] Jesus Uson Minimally Invas Surg Ctr, Stem Cell Therapy Unit, Caceres, Spain
[2] Univ Extremadura, Dept Physiol, Immunol Unit, Caceres, Spain
[3] CIBER Enfermedades Cardiovasc, Caceres, Spain
关键词
exosomes; mesenchymal stem cells; synovitis; immunomodulation; inflammation; AUTOLOGOUS CONDITIONED SERUM; RHEUMATOID-ARTHRITIS; EXTRACELLULAR VESICLES; INTRAARTICULAR INJECTION; TRANSIENT SYNOVITIS; FLUID; OSTEOARTHRITIS; ANGIOGENESIS; IMPROVE; IL-8;
D O I
10.3389/fvets.2017.00039
中图分类号
S85 [动物医学(兽医学)];
学科分类号
090604 [动物药学];
摘要
Synovitis is an inflammatory process associated with pain, disability, and discomfort, which is usually treated with anti-inflammatory drugs or biological agents. Mesenchymal stem cells (MSCs) have been also successfully used in the treatment of inflammatory-related diseases such as synovitis or arthritis. In the last years, the exosomes derived from MSCs have become a promising tool for the treatment of inflammatory-related diseases and their therapeutic effect is thought to be mediated (at least in part) by their immunomodulatory potential. In this work, we aimed to evaluate the anti-inflammatory effect of these exosomes in an antigen-induced synovitis animal model. To our knowledge, this is the first report where exosomes derived from MSCs have been evaluated in an animal model of synovitis. Our results demonstrated a decrease of synovial lymphocytes together with a downregulation of TNF-alpha transcripts in those exosome-treated joints. These results support the immunomodulatory effect of these exosomes and point out that they may represent a promising therapeutic option for the treatment of synovitis.
引用
收藏
页数:10
相关论文
共 73 条
[1]
Comparison of mesenchymal stem cells and leukocytes from Large White and Gottingen Minipigs: Clues for stem cell-based immunomodulatory therapies [J].
Alvarez, Veronica ;
Sanchez-Margallo, Francisco-Miguel ;
Blazquez, Rebeca ;
Tarazona, Raquel ;
Casado, Javier G. .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2016, 179 :63-69
[2]
Pre-rheumatoid arthritis: predisposition and transition to clinical synovitis [J].
Arend, William P. ;
Firestein, Gary S. .
NATURE REVIEWS RHEUMATOLOGY, 2012, 8 (10) :573-586
[3]
Autologous conditioned serum (Orthokine) is an effective treatment for knee osteoarthritis [J].
Baltzer, A. W. A. ;
Moser, C. ;
Jansen, S. A. ;
Krauspe, R. .
OSTEOARTHRITIS AND CARTILAGE, 2009, 17 (02) :152-160
[4]
Roles of exosomes in cardioprotection [J].
Barile, Lucio ;
Moccetti, Tiziano ;
Marban, Eduardo ;
Vassalli, Giuseppe .
EUROPEAN HEART JOURNAL, 2017, 38 (18) :1372-1379
[5]
Anti-TNFα therapy in a cohort of rheumatoid arthritis patients: Clinical outcomes [J].
Bazzani, Chiara ;
Filippini, Matteo ;
Caporali, Roberto ;
Bobbio-Pallavicini, Francesca ;
Favalli, Ennio Giulio ;
Marchesoni, Antonio ;
Atzeni, Fabiola ;
Sarzi-Puttini, Piercarlo ;
Gorla, Roberto .
AUTOIMMUNITY REVIEWS, 2009, 8 (03) :260-265
[6]
Immunomodulatory potential of human adipose mesenchymal stem cells derived exosomes on in vitro stimulated T cells [J].
Blazquez, Rebeca ;
Sanchez-Margallo Francisco, Miguel ;
de la Rosa, Olga ;
Dalemans, Wilfried ;
Alvarez, Veronica ;
Tarazona, Raquel ;
Casado Javier, G. .
FRONTIERS IN IMMUNOLOGY, 2014, 5
[7]
Rheumatoid arthritis: From autoimmunity to synovitis and joint destruction [J].
Boissier, Marie-Christophe ;
Semerano, Luca ;
Challal, Salima ;
Saidenberg-Kermanac'h, Nathalie ;
Falgarone, Geraldine .
JOURNAL OF AUTOIMMUNITY, 2012, 39 (03) :222-228
[8]
Comparative phenotypic and molecular characterization of porcine mesenchymal stem cells from different sources for translational studies in a large animal model [J].
Casado, Javier G. ;
Gomez-Mauricio, G. ;
Alvarez, V. ;
Mijares, J. ;
Tarazona, R. ;
Bernad, A. ;
Sanchez-Margallo, F. M. .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2012, 147 (1-2) :104-112
[9]
Exosomes derived from human menstrual blood-derived stem cells alleviate fulminant hepatic failure [J].
Chen, Lu ;
Xiang, Bingyu ;
Wang, Xiaojun ;
Xiang, Charlie .
STEM CELL RESEARCH & THERAPY, 2017, 8
[10]
Immunomodulatory effects of mesenchymal stromal cells-derived exosome [J].
Chen, Wancheng ;
Huang, Yukai ;
Han, Jiaochan ;
Yu, Lili ;
Li, Yanli ;
Lu, Ziyuan ;
Li, Hongbo ;
Liu, Zenghui ;
Shi, Chenyan ;
Duan, Fengqi ;
Xiao, Yang .
IMMUNOLOGIC RESEARCH, 2016, 64 (04) :831-840