Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity

被引：21

作者：

Aoshima, Hisae ^{[1
]}

Yamana, Shuichi ^{[1
]}

Nakamura, Shigeo ^{[2
]}

Mashino, Tadahiko ^{[2
]}

机构：

[1] Vitamin C60 BioRes Corp, Chuo Ku, Tokyo 1030028, Japan

[2] Keio Univ, Fac Pharm, Dept Pharmaceut Sci, Minato Ku, Tokyo 1058512, Japan

来源：

JOURNAL OF TOXICOLOGICAL SCIENCES | 2010年 / 35卷 / 03期

关键词：

Fullerene; Safety evaluation; Genotoxicity; Phototoxicity; Pro-oxidant; HUMAN SKIN KERATINOCYTES; DOWN-REGULATION; C-60; PHOTOCYTOTOXICITY; MUTAGENICITY; MUTAGENESIS; EXPRESSION; DISEASE; DAMAGE; MOUSE;

D O I：

10.2131/jts.35.401

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe2+ or Cu2+. Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.

引用

页码：401 / 409

页数：9

共 19 条

[1]

Safety evaluation of highly purified fullerenes (HPFs): based on screening of eye and skin damage [J].

Aoshima, Hisae ;

Saitoh, Yasukazu ;

Ito, Shinobu ;

Yamana, Shuichi ;

Miwa, Nobuhiko .

JOURNAL OF TOXICOLOGICAL SCIENCES, 2009, 34 (05) :555-562

[2]

Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis [J].

Basso, Alexandre S. ;

Frenkel, Dan ;

Quintana, Francisco J. ;

Costa-Pinto, Frederico A. ;

Petrovic-Stojkovic, Sanja ;

Puckett, Lindsay ;

Monsonego, Alon ;

Bar-Shir, Amnon ;

Engel, Yon ;

Gozin, Michael ;

Weiner, Howard L. .

JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (04) :1532-1543

[3]

Polyhydroxylated Fullerene Derivative C60(OH)24 Prevents Mitochondrial Dysfunction and Oxidative Damage in an MPP+-Induced Cellular Model of Parkinson's Disease [J].

Cai, Xiaoqing ;

Jia, Haiqun ;

Liu, Zhongbo ;

Hou, Bei ;

Luo, Cheng ;

Feng, Zhihui ;

Li, Wenxin ;

Liu, Jiankang .

JOURNAL OF NEUROSCIENCE RESEARCH, 2008, 86 (16) :3622-3634

[4]

Gene expression profiles in rat lung after inhalation exposure to C60 fullerene particles [J].

Fujita, Katsuhide ;

Morimoto, Yasuo ;

Ogami, Akira ;

Myojyo, Toshihiko ;

Tanaka, Isamu ;

Shimada, Manabu ;

Wang, Wei-Ning ;

Endoh, Shigehisa ;

Uchida, Kunio ;

Nakazato, Tetsuya ;

Yamamoto, Kazuhiro ;

Fukui, Hiroko ;

Horie, Masanori ;

Yoshida, Yasukazu ;

Iwahashi, Hitoshi ;

Nakanishi, Junko .

TOXICOLOGY, 2009, 258 (01) :47-55

[5]

MUTAGEN TESTING USING TRP+ REVERSION IN ESCHERICHIA-COLI [J].

GREEN, MHL ;

MURIEL, WJ .

MUTATION RESEARCH, 1976, 38 (01) :3-32

[6]

Biological safety of liposome-fullerene consisting of hydrogenated lecithin, glycine soja sterols, and fullerene-C60 upon photocytotoxicity and bacterial reverse mutagenicity [J].

Kato, S. ;

Aoshima, H. ;

Saitoh, Y. ;

Miwa, N. .

TOXICOLOGY AND INDUSTRIAL HEALTH, 2009, 25 (03) :197-203

[7]

Biological Safety of LipoFullerene composed of Squalane and Fullerene-C60 upon Mutagenesis, Photocytotoxicity, and Permeability into the Human Skin Tissue [J].

Kato, Shinya ;

Aoshima, Hisae ;

Saitoh, Yasukazu ;

Miwa, Nobuhiko .

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2009, 104 (06) :483-487

[8]

C-60 - BUCKMINSTERFULLERENE [J].

KROTO, HW ;

HEATH, JR ;

OBRIEN, SC ;

CURL, RF ;

SMALLEY, RE .

NATURE, 1985, 318 (6042) :162-163

[9]

REVISED METHODS FOR THE SALMONELLA MUTAGENICITY TEST [J].

MARON, DM ;

AMES, BN .

MUTATION RESEARCH, 1983, 113 (3-4) :173-215

[10]

C-60 AS A RADICAL SPONGE [J].

MCEWEN, CN ;

MCKAY, RG ;

LARSEN, BS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (11) :4412-4414

← 1 2 →