Targeted high-throughput sequencing of tagged nucleic acid samples

被引:138
作者
Meyer, Matthias [1 ]
Stenzel, Udo [1 ]
Myles, Sean [1 ]
Pruefer, Kay [1 ]
Hofreiter, Michael [1 ]
机构
[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany
关键词
D O I
10.1093/nar/gkm566
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.
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页数:5
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