Experimental parameters differentially affect the humoral response of the cholera-toxin-based murine model of food allergy

被引:18
作者
Kroghsbo, S [1 ]
Christensen, HR [1 ]
Frokiær, H [1 ]
机构
[1] Tech Univ Denmark, BioCtr, DK-2800 Lyngby, Denmark
关键词
cholera toxin; animal model; food allergy; antigen-specific IgE; ELISA;
D O I
10.1159/000072137
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Recent studies have developed a murine model of IgE-mediated food allergy based on oral coadministration of antigen and cholera toxin (CT) to establish a maximal response for studying immunopathogenic mechanisms and immunotherapeutic strategies. However, for studying subtle immunomodulating factors or factors effective during response initiation, this maximal response-based model is less suitable due to a lack of sensitivity. Therefore, in attempts to identify essential parameters to fine-tune the immune response towards a submaximal level, potentially more sensitive, we were interested in characterizing the individual effects of the parameters in the CT-based model: CT dose, antigen type and dose, and number of immunizations. Methods: BALB/c mice were orally sensitized weekly for 3 or 7 weeks with graded doses of CT and various food antigens (soy-trypsin inhibitor, ovalbumin or ovomucoid). Antigen-specific lgG1, IgG2a, IgA and IgE were monitored by ELISA. Results: The CT dose exerted a clear dose-dependent effect on the antigen-specific antibody response whereas the antigen dose tended to affect the kinetics of the developing response. Both the intensity and kinetics of the antibody response depended on the type of antigen and number of immunizations. Conclusions: The critical parameters of the CT-based murine allergy model differentially control the intensity and kinetics of the developing immune response. Adjustment of these parameters could be a key tool for tailoring the response to submaximal levels rendering the model potentially more sensitive for evaluating the effect of subtle immunomodulating factors that would be lost in the maximal response-based model. Copyright (C) 2003 S. Karger AG, Basel.
引用
收藏
页码:256 / 263
页数:8
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