Scaling up the ligase chain reaction-based approach to gene synthesis

被引：10

作者：

Chalmers, FM

Curnow, KM

机构：

[1] Baker Med Res Inst, Melbourne, Vic 8008, Australia

[2] Monash Univ, Melbourne, Vic 3004, Australia

来源：

BIOTECHNIQUES | 2001年 / 30卷 / 02期

关键词：

D O I：

10.2144/01302bm02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：249 / +

页数：3

共 8 条

[1] Gene synthesis by a LCR-based approach:: High-level production of leptin-L54 using synthetic gene in Escherichia coli [J].

Au, LC ;

Yang, FY ;

Yang, WJ ;

Lo, SH ;

Kao, CF .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 248 (01) :200-203

[2] GENE SYNTHESIS, HIGH-LEVEL EXPRESSION, AND MUTAGENESIS OF THIOBACILLUS-FERROOXIDANS RUSTICYANIN - HIS-85 IS A LIGAND TO THE BLUE COPPER CENTER [J].

CASIMIRO, DR ;

TOYPALMER, A ;

BLAKE, RC ;

DYSON, HJ .

BIOCHEMISTRY, 1995, 34 (20) :6640-6648

[3] PCR-based gene synthesis and protein NMR spectroscopy [J].

Casimiro, DR ;

Wright, PE ;

Dyson, HJ .

STRUCTURE, 1997, 5 (11) :1407-1412

[4] PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases [J].

Cline, J ;

Braman, JC ;

Hogrefe, HH .

NUCLEIC ACIDS RESEARCH, 1996, 24 (18) :3546-3551

[5] BACULOVIRUS EXPRESSION OF HUMAN BASIC FIBROBLAST GROWTH-FACTOR FROM A SYNTHETIC GENE - ROLE OF THE KOZAK CONSENSUS AND COMPARISON WITH BACTERIAL EXPRESSION [J].

HILLS, D ;

CRANEROBINSON, C .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1995, 1260 (01) :14-20

[6] Expression and codon usage optimization of the erythroid-specific transcription factor cGATA-1 in baculoviral and bacterial systems [J].

Pikaart, MJ ;

Felsenfeld, G .

PROTEIN EXPRESSION AND PURIFICATION, 1996, 8 (04) :469-475

[7] RECURSIVE PCR - A NOVEL TECHNIQUE FOR TOTAL GENE SYNTHESIS [J].

PRODROMOU, C ;

PEARL, LH .

PROTEIN ENGINEERING, 1992, 5 (08) :827-829

[8] RAPID EVOLUTION OF A PROTEIN IN-VITRO BY DNA SHUFFLING [J].

STEMMER, WPC .

NATURE, 1994, 370 (6488) :389-391

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