Clinical analysis of human urinary proteins using high resolution electrophoretic methods

被引：39

作者：

Marshall, T ^{[1
]}

Williams, KM ^{[1
]}

机构：

[1] Univ Sunderland, Sch Hlth Sci, Analyt Biochem Grp, Sunderland SR1 3RG, England

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 10期

关键词：

urinary proteins; isoelectric focusing; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; two-dimensional polyacrylamide gel electrophoresis; capillary electrophoresis;

D O I：

10.1002/elps.1150191037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of isoelectric focusing (IEF), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional electrophoresis (2-DE) and capillary electrophoresis (CE) for high resolution electrophoretic analysis of human urinary proteins is reviewed. In each case, the information is tabulated chronologically with details of sample preparation, electrophoretic system, detection method and clinical application. The text includes an historical perspective of the use of each method for urinalysis and a detailed review of the application of the methods to the investigation of renal disease, renal transplantation, Bence Jones proteinuria (BJP), diabetes mellitus, cadmium toxicity, nephrolithiasis and cancers of the urogenital tract.

引用

页码：1752 / 1770

页数：19

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