Configuration and racemization determination of cysteine residues in peptides by chiral derivatization and HPLC:: Application to oxytocin peptides

被引：13

作者：

Szabó, S

Szókán, G

Khlafulla, AM

Almás, M

Kiss, C

Rill, A

Schön, I

机构：

[1] Eotvos Lorand Univ, Dept Organ Chem, H-1518 Budapest 112, Hungary

[2] Gedeon Richter Chem Works Ltd, Chem Works, Budapest, Hungary

来源：

JOURNAL OF PEPTIDE SCIENCE | 2001年 / 7卷 / 06期

关键词：

chiral derivatization; cysteine; HPLC; racemization;

D O I：

10.1002/psc.325

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved RP-HPLC method was developed for the determination of the configuration and stereochemical purity of cysteine residues in peptides. The method consists of oxidation of cysteine and cystine residues to cysteic acid, followed by hydrolysis and pre-column chiral derivatization with Val-Marfey's reagent. Copyright (C) 2001 European Peptide Society and John Wiley & Sons, Ltd.

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收藏

页码：316 / 322

页数：7

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