Affinity purification of DNA-binding proteins

被引：22

作者：

Gadgil, H ^{[1
]}

Oak, SA ^{[1
]}

Jarrett, HW ^{[1
]}

机构：

[1] Univ Tennessee, Dept Biochem, Memphis, TN 38163 USA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

DNA-binding proteins; purification; DNA affinity chromatography;

D O I：

10.1016/S0165-022X(01)00223-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed. The purification strategies for transcription factors, restriction enzymes, telomerases, DNA and RNA polymerase and DNA binding antibodies are described. Different applications of DNA affinity chromatography are presented. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：607 / 624

页数：18

共 81 条

[1]

Alberts B., 1971, Methods Enzymol, V21, P198

[2]

STUDIES WITH DNA-CELLULOSE CHROMATOGRAPHY .I. DNA-BINDING PROTEINS FROM ESCHERICHIA COLI [J].