Improvement of ACE inhibitory activity of chitooligosaccharides (COS) by carboxyl modification

被引：62

作者：

Huang, R

Mendis, E

Kim, SK ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[2] Wuhan Univ, Dept Water Chem, Wuhan 430072, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2005年 / 13卷 / 11期

关键词：

chitooligosaccharides (COS); angiotensin I converting enzyme (ACE); carboxylated COS; quartenized amino COS; competitive inhibitor;

D O I：

10.1016/j.bmc.2005.03.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In the present research, chitooligosaccharides (COS) were carboxylated with -COCH2CH2COO- groups to obtain specific structural features similar to Captopril((R)). Angiotensin I converting enzyme (ACE) inhibitory activity of carboxylated COS was studied and observed to enhance its activity with increased substitution degree. Further, Lineweaver-Burk plot analysis revealed that inhibition was competitive via obligatory binding site of the enzyme. This was accompanied with substitution of positively charged quarternized amino groups to COS with different substitution degrees, in which negative impact on ACE inhibition was observed. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：3649 / 3655

页数：7

共 23 条

[1]

Inhibition of angiotensin converting enzyme (ACE) activity by flavan-3-ols and procyanidins [J].