Enzymatic synthesis of amorphous calcium phosphate -: Chitosan nanocomposites and their processing into hierarchical structures

Hierarchically organized Amorphous Calcium Phosphate (ACP)/CHI hydrogel nanocomposites were prepared for ISISA processing by an enzymatically assisted route that causes ACP-NP precipitation and CHI gelation concurrently. The calcination of hierarchically organized ACP/CHI materials provides the formation of inorganic hierarchically organized materials, such as HA-NP hierarchical structures in which individual HA nanocrystals aggregate while keeping the macroporous structures. ISISA process also allows for control of the macroporous size from 25 upto 90 micromilimeter for HA-NP hierarchical structures. The morphology of resulting hierarchical structures, such as microsponge-like besides the biocompatible chemistry involved in their formation, should make these materials very interesting for tissue engineering purposes.