The Expression Analysis of Nanog in the Developing Rat Myocardial Tissues

被引：5

作者：

Guo, Zhi-kun ^{[1
]}

Guo, Kang ^{[2
]}

Luo, Huanhuan ^{[1
]}

Mu, Ling-min ^{[1
]}

Li, Qiong ^{[1
]}

Chang, Yu-qiao ^{[1
]}

机构：

[1] Xinxiang Med Univ, Affiliated Hosp 3, Key Lab Med Tissue Regenerat Henan Prov, Xinxiang City, Peoples R China

[2] Xinxiang Med Univ, Affiliated Hosp 3, Tumor Dept, Xinxiang City, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 35卷 / 03期

关键词：

Nanog; Immunohistochemistry; Western blotting; RT-PCR; Myocardial tissue; Rat heart; EMBRYONIC STEM-CELLS; SELF-RENEWAL; PLURIPOTENCY; MOUSE; TRANSCRIPTION; REGENERATION; CLONING; HEART;

D O I：

10.1159/000369744

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Objectives: To investigate the expression dynamic of nanog gene in the development of rat myocardial tissues. Methods: SD rats were studied at 5 time points before and after birth. The techniques of immunohistochemistry, immunofluorescence, western blotting and RT-PCR were used to investigate the expression of nanog gene in the rat myocardial tissues at different embryonic (E) and postnatal (P) stages, and image analysis system was used for the quantitative analysis. Results: The immunohistochemistry, immunofluorescence and western blotting analyses have shown that expression of nanog protein was highest in the rat myocardial tissues at E18, then it gradually declined at postnatal stages (P < 0.05), and became nearly undetectable in most myocardial tissues at P30 with very few remaining nanogpositive cells. RT-PCR result indicated that the expression of nanog gene was strong at E18, but gradually decreased from E18 to P30. Conclusion: The mRNA transcription and protein translation of nanog gene in the rat heart gradually decreased with every consecutive growth stage. This indicates that nanog gene has potential regulatory functions in the differentiation of myocardial cells during rat development. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：866 / 874

页数：9

共 20 条

[1]

Adult cardiac stem cells are multipotent and support myocardial regeneration [J].