A novel enzyme system for the reduction of 3-oxo bile acids in human red blood cells

被引：3

作者：

Goto, J ^{[1
]}

Miura, H ^{[1
]}

Ando, N ^{[1
]}

Yamato, Y ^{[1
]}

Ikegawa, S ^{[1
]}

Nambara, T ^{[1
]}

Makino, I ^{[1
]}

机构：

[1] ASAHIKAWA MED COLL,DEPT INTERNAL MED 2,ASAHIKAWA,HOKKAIDO 078,JAPAN

来源：

STEROIDS | 1996年 / 61卷 / 07期

关键词：

3-oxo bile acid; enzymic reduction; extrahepatic biotransformation; red blood cell; gas chromatography mass spectrometry; ion cluster technique;

D O I：

10.1016/0039-128X(96)00061-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

7 alpha,12 alpha-Dihydroxy-3-oxo- and 3,7,12-trioxo-5 beta-cholanoic acids labeled with O-18 atoms were incubated with human red blood cells, and the biotransformation products were separated and characterized by gas chromatography-mass spectrometry as the pentafluorobenzyl ester-trimethylsilyl and -dimethylethylsilyl ether derivatives with the negative ion chemical ionization mode. The reduced products, 3 beta,7 alpha-12 alpha-trihydroxy-5 beta-cholanoic acid for the former, and 3 alpha-hydroxylated dioxo bile acid together with 3 beta-hydroxylated 7,12-dioxo-5 beta-cholanoic acid for the latter, were identified as metabolites. When 3-oxo bile acid was incubated with human blood denatured at 70 degrees C for 2 min, no metabolites were formed. The enzymic reduction activity has been localized in the red blood cell fraction.

引用

页码：416 / 420

页数：5

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