The Severity of Dextran Sodium Sulfate-Induced Colitis Can Differ Between Dextran Sodium Sulfate Preparations of the Same Molecular Weight Range

被引：29

作者：

Bamba, Shigeki ^{[1
]}

Andoh, Akira ^{[2
]}

Ban, Hiromitsu ^{[1
]}

Imaeda, Hirotsugu ^{[1
]}

Aomatsu, Tomoki ^{[3
]}

Kobori, Ayako ^{[1
]}

Mochizuki, Yosuke ^{[4
]}

Shioya, Makoto ^{[1
]}

Nishimura, Takashi ^{[1
]}

Inatomi, Osamu ^{[1
]}

Sasaki, Masaya

Saitoh, Yasuharu ^{[4
]}

Tsujikawa, Tomoyuki

Araki, Yoshio

Fujiyama, Yoshihide ^{[1
]}

机构：

[1] Shiga Univ Med Sci, Div Gastroenterol, Otsu, Shiga 5202192, Japan

[2] Shiga Univ Med Sci, Grad Sch Med, Div Mucosal Immunol, Otsu, Shiga 5202192, Japan

[3] Osaka Med Coll, Dept Pediat, Takatsuki, Osaka 569, Japan

[4] Shiga Univ Med Sci, Div Endoscopy, Otsu, Shiga 5202192, Japan

来源：

DIGESTIVE DISEASES AND SCIENCES | 2012年 / 57卷 / 02期

关键词：

Inflammatory bowel disease; Dextran sulfate; Colitis; PERFORMANCE LIQUID-CHROMATOGRAPHY; ULCERATIVE-COLITIS; IMMUNODEFICIENT MICE; LABORATORY-ANIMALS; GROWTH-FACTOR; GUINEA-PIGS; COLON; DISEASE; LIGNOSULFONATE; APOPTOSIS;

D O I：

10.1007/s10620-011-1881-x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

We hypothesized that the severity of dextran sodium sulfate (DSS)-induced colitis could differ between DSS preparations of the same molecular weight, and that this difference may be affected by the sulfur content. To test this, we used three DSS preparations of similar molecular weights but with different sulfur contents. Three DSS preparations with molecular weights of 40,000 to 50,000 were tested: MP Biomedicals (MP Bio), USB (USB), and The Lab Depot (The Lab). Epithelial cell lines were used to assess the levels of poly (ADP-ribose) polymerase (PARP) in the presence of 2.0% DSS in vitro. Eight-week-old female C57/B6 mice were fed 2.0% DSS in water for 1 week, and then sacrificed to investigate the effects of the DSS preparations in vivo. In vitro experiments using CaCo-2 and CMT-93 cells revealed decreased PARP levels from all DSS preparations. Notably, the PARP level was significantly decreased in CaCo-2 cells treated with DSS from USB as compared to The Lab Mice treated with The Lab DSS had significantly decreased body weight losses on day 7 as compared to mice receiving DSS from MP Bio and USB. This result was supported by their DAI score, colon weight/length ratio, and histological scores. The severity of colitis can differ between similar DSS preparations of the same molecular weight range. This difference in colitogenic properties may be affected by the total sulfur content of each DSS preparation.

引用

页码：327 / 334

页数：8

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