The Pla surface protease/adhesin of Yersinia pestis mediates bacterial invasion into human endothelial cells

被引:72
作者
Lähteenmäki, K [1 ]
Kukkonen, M [1 ]
Korhonen, TK [1 ]
机构
[1] Univ Helsinki, Div Gen Microbiol, Dept Biosci, FIN-00014 Helsinki, Finland
基金
芬兰科学院;
关键词
Pla; plague; invasion; endothelial cell; Yersinia;
D O I
10.1016/S0014-5793(01)02775-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plasminogen activator Pla of Yersinia pestis belongs to the omptin family of enterobacterial surface proteases and is responsible for the highly efficient invasion of the plague bacterium from the subcutaneous infection site into the circulation. Y. pestis has been reported to invade human epithelial cells. Here, we investigated the role of Pla in bacterial invasion into human endothelial cells. Expression of Pla in recombinant Escherichia coli XL1(pMRK1) enhanced bacterial invasion into ECV304 cells. The invasiveness was not affected by substitution mutation at the residues S99 or D206 that are needed for the proteolytic activity of Pla. Pla-expressing bacteria adhered to the extracellular matrix of ECV304 cells. Only weak adhesion and poor invasion were seen with the recombinant E. coli XL1(pMRK2), which expresses the omptin homolog from E. coli. The results identify Pla as an invasion protein of Y. pestis and show that the invasive function does not involve the proteolytic activity of Pla. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:69 / 72
页数:4
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