Poly(2-dimethylamino ethylmethacrylate)-Based Polymers To Camouflage Red Blood Cell Antigens

被引：8

作者：

Cerda-Cristerna, Bernardino Isaac ^{[1
,2
]}

Cottin, Sophie ^{[1
]}

Flebus, Luca ^{[1
]}

Pozos-Guillen, Amaury ^{[2
]}

Flores, Hector ^{[2
]}

Heinen, Ernst ^{[3
]}

Jolois, Olivier ^{[3
]}

Gerard, Christiane ^{[4
]}

Maggipinto, Gianni ^{[4
]}

Sevrin, Chantal ^{[1
]}

Grandfils, Christian ^{[1
]}

机构：

[1] Univ Liege, Interfacultary Res Ctr Biomat, Inst Chem, B-4000 Liege, Belgium

[2] Univ San Luis Potosi, Lab Basic Sci, Fac Dent, San Luis Potosi 78290, Mexico

[3] Univ Liege, Biomed & Preclin Sci Dept, Human Histol Lab, B-4000 Liege, Belgium

[4] Univ Liege, Hematol & Immunohematol Dept, Cent Hosp, B-4000 Liege, Belgium

来源：

BIOMACROMOLECULES | 2012年 / 13卷 / 04期

关键词：

ELECTROPHORETIC MOBILITY; POLYCATIONS; STABILITY; HEMOCOMPATIBILITY; AGGLUTINATION; ADSORPTION; ANTIBODIES; MEMBRANE;

D O I：

10.1021/bm300127f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane surface of red blood cells (RBCs). The electrostatic self-assembly of PDMAEMA on RBCs membrane can be employed for inducing the formation of a polymeric shield camouflaging blood group antigens on RBCs as a valuable strategy for developing "universal RBCs" for blood transfusion. The purpose of this research was to evaluate the camouflaging ability of PDMAEMA homopolymers and PDMAEMA-co-poly(ethylene glycol) copolymers differing in molecular weight and architecture. Surprisingly, the PDMAEMAs caused a partially masking, no masking, and sensitization of the same RBCs population. The MW and architecture of the polymers as well as temperature of PDMAEMA-RBCs treatment influenced the results observed. Herein, the very particular reactivity of PDMAEMAs and RBCs is analyzed and discussed.

引用

页码：1172 / 1180

页数：9

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