Cloning of cDNAs encoding neurotoxic peptides from the spider Phoneutria nigriventer

被引：28

作者：

Kalapothakis, E ^{[1
]}

Penaforte, CL ^{[1
]}

Beirao, PSL ^{[1
]}

Romano-Silva, MA ^{[1
]}

Cruz, JS ^{[1
]}

Prado, MAM ^{[1
]}

Guimaraes, PEM ^{[1
]}

Gomez, MV ^{[1
]}

Prado, VF ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Farmacol, BR-31270901 Belo Horizonte, MG, Brazil

来源：

TOXICON | 1998年 / 36卷 / 12期

关键词：

D O I：

10.1016/S0041-0101(98)00105-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A cDNA library made from venom glands of the spider Phoneutria nigriventer was constructed and used to clone neurotoxic peptides. A cDNA of about 360 nucleotides encoding the precursor for the toxin Tx2-1 active on mammals has been isolated. The deduced amino acid sequence for the mature polypeptide confirms the polypeptide sequence previously published. In addition, two new putative toxins called Pn2-1A and Pn2-5A have been characterized and their complete amino acid sequence show 92% similarity to Tx2-1 and 94% similarity to 7x2-5 respectively. The cDNAs revealed that the precursors contain signal peptides characterized by a very hydrophobic core and a propeptide interposed between the signal sequence and the peptide toxin.. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1843 / 1850

页数：8

共 23 条

[1]

ALTSCHUL SF, 1990, P NATL ACAD SCI USA, V90, P4338

[2]

ARAUJO DAM, 1993, N-S ARCH PHARMACOL, V347, P205

[3]

ARAUJO DAM, 1993, J PHYSIOL-LONDON, V467, pP365

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5] PRECURSOR STRUCTURE OF OMEGA-CONOTOXIN GVIA DETERMINED FROM A CDNA CLONE [J].

COLLEDGE, CJ ;

HUNSPERGER, JP ;

IMPERIAL, JS ;

HILLYARD, DR .

TOXICON, 1992, 30 (09) :1111-1116

[6] PURIFICATION AND AMINO-ACID-SEQUENCES OF 6 TX3-TYPE NEUROTOXINS FROM THE VENOM OF THE BRAZILIAN ARMED SPIDER PHONEUTRIA-NIGRIVENTER (KEYS) [J].

CORDEIRO, MD ;

DEFIGUEIREDO, SG ;

VALENTIM, AD ;

DINIZ, CR ;

VONEICKSTEDT, VRD ;

GILROY, J ;

RICHARDSON, M .

TOXICON, 1993, 31 (01) :35-42

[7] THE PURIFICATION AND AMINO-ACID-SEQUENCES OF 4 TX2 NEUROTOXINS FROM THE VENOM OF THE BRAZILIAN ARMED SPIDER PHONEUTRIA-NIGRIVENTER (KEYS) [J].

CORDEIRO, MD ;

DINIZ, CR ;

VALENTIM, AD ;

VONEICKSTEDT, VRD ;

GILROY, J ;

RICHARDSON, M .

FEBS LETTERS, 1992, 310 (02) :153-156

[8]

CORDEIRO MDN, 1995, J TOXICOL-TOXIN REV, V14, P309, DOI 10.3109/15569549509019466

[9]

DINIZ MRV, 1993, J BIOL CHEM, V268, P15340

[10]

FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266

← 1 2 3 →