Fibrous and helical calcite crystals induced by synthetic polypeptides containing O-phospho-L-serine and O-phospho-L-threonine

被引：24

作者：

Hayashi, Shinya ^{[1
]}

Ohkawa, Kousaku ^{[1
]}

Suwa, Yukie ^{[1
]}

Sugawara, Tetsunori ^{[1
]}

Asami, Takahiro ^{[2
]}

Yamamoto, Hiroyuki ^{[1
]}

机构：

[1] Shinshu Univ, Fac Text Sci & Technol, Inst High Polymer Res, Ueda, Nagano 3868567, Japan

[2] Shinshu Univ, Fac Sci, Dept Biol, Matsumoto, Nagano 3908621, Japan

来源：

MACROMOLECULAR BIOSCIENCE | 2008年 / 8卷 / 01期

关键词：

biomineralization; calcium carbonate; crystal growth; O-phospho-L-serine; O-phospho-L-threonine; polypeptides;

D O I：

10.1002/mabi.200700096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The modification of CaCO3 crystal growth by synthetic L-Ser(PO3H2) and L-Thr(PO3H2) containing polypeptides is described. The amino acids Gly, L-GlU, L-Asp, L-Ser, L-Ala, and L-Lys induced rhombohedral calcite with a rough surface. Dipeptides, Xaa-L-Ser(PO3H2) (Xaa = Gly, L-Glu, L-Asp, L-Ser, L-Ala and L-Lys) induced vaterite crystals in the lower [Ca2+]. on the other hand, L-Ser(PO3H2)-containing polypeptides formed spherical vaterite and fibrous calcite. The characteristic helical calcite was found in the presence of copoly[L-Ser(PO3H2)(75) L-Asp(25)] or poly[L-Ser(PO3H2)(3)-L-Asp]. Fibrous calcite, spherical vaterite, and helical calcite crystals were subjected to XRD and EDX analysis. XRD revealed the specific faces of these crystals. EDX spectra-and surface analysis visualized the localization of the polypeptides and CaCO3 components. Together with TEM and SAED data, we propose hypothetical growth mechanisms for the fibrous and helical calcite crystals. [GRAPHICS]

引用

页码：46 / 59

页数：14

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