Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology

被引：33

作者：

Ratnam, BVV

Rao, SS

Rao, MD

Rao, MN

Ayyanna, C

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pediat, Div Infect Dis, Baltimore, MD 21205 USA

[3] Andhra Univ, Coll Engn, Dept Chem Engn, Ctr Biotechnol, Waltair 530003, Andhra Pradesh, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2005年 / 21卷 / 04期

关键词：

central composite design (CCD); ethanol; palmyra jaggery; response surface methodology (RSM); Saccharomyces cerevisiae;

D O I：

10.1007/s11274-004-2461-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The quantitative effects of sugar concentration, nitrogen concentration, EDTA, temperature, pH and time of fermentation on ethanol production were optimized using a Box-Wilson central composite design (CCD) experiment. It was found that palmyra jaggery (sugar syrup from the palmyra palm) is a suitable substrate for the production of high concentrations of ethanol using Saccharomyces cerevisiae NCIM 3090 by submerged fermentation. A maximum ethanol concentration of 129.4 g/l was obtained after optimizing media components and conditions of fermentation. The optimum values were a temperature of 26.2 degrees C , pH of 8.4, time of fermentation of 4.2 days with 398.5 g of substrate/l, 3.1 g of urea/l and 0.51 g of EDTA/l. Thus by using the CCD, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.

引用

页码：399 / 404

页数：6

共 16 条

[1] Optimizing medium constituents and fermentation conditions for citric acid production from palmyra jaggery using response surface method [J].