The 35S promoter used in a selectable marker gene of a plant transformation vector affects the expression of the transgene

被引:121
作者
Yoo, SY
Bomblies, K
Yoo, SK
Yang, JW
Choi, MS
Lee, JS
Weigel, D
Ahn, JH [1 ]
机构
[1] Korea Univ, Sch Life Sci & Biotechnol, Plant Signaling Network Res Ctr, Seoul 136701, South Korea
[2] Seoul Natl Univ, Sch Biol Sci, Seoul 152742, South Korea
[3] Max Planck Inst Dev Biol, Dept Biol Mol, D-72076 Tubingen, Germany
[4] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
关键词
35S enhancer; 35S promoter; misexpression; plant transformation vector; trans activation;
D O I
10.1007/s00425-004-1466-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Positive selection of transgenic plants is essential during plant transformation. Thus, strong promoters are often used in selectable marker genes to ensure successful selection. Many plant transformation vectors, including pPZP family vectors, use the 35S promoter as a regulatory sequence for their selectable marker genes. We found that the 35S promoter used in a selectable marker gene affected the expression pattern of a transgene, possibly leading to a misinterpretation of the result obtained from transgenic plants. It is likely that the 35S enhancer sequence in the 35S promoter is responsible for the interference, as in the activation tagging screen. This affected expression mostly disappeared in transgenic plants generated using vectors without the 35S sequences within their T-DNA region. Therefore, we suggest that caution should be used in selecting a plant transformation vector and in the interpretation of the results obtained from transgenic approaches using vectors carrying the 35S promoter sequences within their T-DNA regions.
引用
收藏
页码:523 / 530
页数:8
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